Loss of C/EBP alpha cell cycle control increases myeloid progenitor proliferation and transforms the neutrophil granulocyte lineage
CCAAT/enhancer binding protein (C/EBP)alpha is a myeloid-specific transcription factor that couples lineage commitment to terminal differentiation and cell cycle arrest, and is found mutated in 9% of patients who have acute myeloid leukemia (AML). We previously showed that mutations which dissociate...
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Published in | The Journal of experimental medicine Vol. 202; no. 1; pp. 85 - 96 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
The Rockefeller University Press
04.07.2005
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Subjects | |
Online Access | Get full text |
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Summary: | CCAAT/enhancer binding protein (C/EBP)alpha is a myeloid-specific transcription factor that couples lineage commitment to terminal differentiation and cell cycle arrest, and is found mutated in 9% of patients who have acute myeloid leukemia (AML). We previously showed that mutations which dissociate the ability of C/EBP alpha to block cell cycle progression through E2F inhibition from its function as a transcriptional activator impair the in vivo development of the neutrophil granulocyte and adipose lineages. We now show that such mutations increase the capacity of bone marrow (BM) myeloid progenitors to proliferate, and predispose mice to a granulocytic myeloproliferative disorder and transformation of the myeloid compartment of the BM. Both of these phenotypes were transplantable into lethally irradiated recipients. BM transformation was characterized by a block in granulocyte differentiation, accumulation of myeloblasts and promyelocytes, and expansion of myeloid progenitor populations--all characteristics of AML. Circulating myeloblasts and hepatic leukocyte infiltration were observed, but thrombocytopenia, anemia, and elevated leukocyte count--normally associated with AML-were absent. These results show that disrupting the cell cycle regulatory function of C/EBP alpha is sufficient to initiate AML-like transformation of the granulocytic lineage, but only partially the peripheral pathology of AML. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Abbreviations used: AML, acute myeloid leukemia; BFU-E, burst-forming units–erythrocyte; BRM, basic region mutant; C/EBP, CCAAT/enhancer binding protein; CMP, common myeloid progenitor; G-CSF-R, granulocyte colony-stimulating factor receptor; GEMM, granulocyte/erythrocyte/macrophage/megakaryocyte; GM, granulocyte/macrophage; GMP, granulocyte/macrophage progenitor; HSC, hematopoietic stem cell; M-CSF-R, macrophage colony-stimulating factor receptor. CORRESPONDENCE Claus Nerlov: nerlov@embl-monterotondo.it |
ISSN: | 0022-1007 1540-9538 1540-9538 1892-1007 |
DOI: | 10.1084/jem.20050067 |