Isolation and identification of Sphingomonas sp. that yields tert-octylphenol monoethoxylate under aerobic conditions

• Published in: Bioscience, biotechnology, and biochemistry Vol. 69; no. 7; pp. 1226 - 1231
• Main Authors: Nishio, E. (Kyushu Kyoritsu Univ., Kitakyushu, Fukuoka (Japan)), Yoshikawa, H, Wakayama, M, Tamura, H, Morita, S, Tomita, Y
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.07.2005; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: AEROBIOSIS; Aluminum Silicates - pharmacology; BACTERIA; BIODEGRADATION; Biodegradation, Environmental - drug effects; Biological and medical sciences; Chromatography, High Pressure Liquid; CULTURE TECHNIQUES; DNA, Bacterial - genetics; ENDOCRINE DISEASES; endocrine disrupter; ETHYL ETHER; Ethyl Ethers - chemistry; Ethyl Ethers - metabolism; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry; IDENTIFICATION; ISOLATION; Molecular Structure; NUCLEOTIDE SEQUENCE; Phenols - chemistry; Phenols - metabolism; Phylogeny; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; RNA, Ribosomal, 16S - genetics; Soil Microbiology; Soil Pollutants - analysis; Soil Pollutants - metabolism; Sphingomonas - genetics; Sphingomonas - isolation & purification; Sphingomonas - metabolism; tert-octylphenol monoethoxylate; Biodegradation; endocrine disrupter; Bacteria; Isolation; Sphingomonas; Identification; Endocrine disruptor; Yield; Aerobe; tert-octylphenol monoethoxylate
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.69.1226

Topsoil samples were collected from eight golf courses in Yamaguchi Prefecture, Japan, and enrichment cultures were carried out with a basal-salt medium containing 0.2% 4-tert-octylphenol polyethoxylate (OPPEO) as sole carbon source. OPPEO-degrading activity was detected in one of the samples, from which a strain of OPPEO-degrading bacterium was isolated. The isolated bacterium grew on a nutritionally enriched medium (NE medium) containing 0.2% OPPEO as sole carbon source, and accumulated 4-tert-octylphenol diethoxylate (OP2EO) (63%), 4-tert-octylphenol triethoxylate (OP3EO) (14%), and 4-tert-octylphenol monoettaoxylate (OP1EO) (2%) after 7d cultivation under aerobic conditions. The addition of clay mineral (vermiculite) to the medium accelerated the degradation of OP2EO (40%) and OP3EO (4%) to OP1EO (23%). This is the first report about bacteria that can degrade OPPEO to OP1EO under aerobic conditions. The strain was identified as Sphingomonas macrogohabidus, based on the homology of a 16S rDNA sequence.