Gelatin-Alginate Complexes for EGF Encapsulation: Effects of H-Bonding and Electrostatic Interactions

Gelatin Type A (GA) and sodium alginate (SA) complexes were explored to encapsulate epidermal growth factor (EGF), and thereby to circumvent its proteolytic degradation upon topical application to chronic wounds. Phase diagrams were constructed based on turbidity as a function of GA to SA ratio and...

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Published inPharmaceutics Vol. 11; no. 10; p. 530
Main Authors Jeong, Seonghee, Kim, ByungWook, Lau, Hui-Chong, Kim, Aeri
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 14.10.2019
MDPI
Subjects
Acids
alginate
Antibodies
coacervate
diabetic foot ulcer
Efficiency
electrostatic interactions
Epidermal growth factor
epidermal growth factor (egf)
Fibroblasts
Fourier transforms
gelatin
h-bonding interactions
Molecular weight
Pharmaceutical industry
polyelectrolyte complex
Polymers
Proteins
Spectrum analysis
United Kingdom > UK
United States > US
Japan
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Summary:Gelatin Type A (GA) and sodium alginate (SA) complexes were explored to encapsulate epidermal growth factor (EGF), and thereby to circumvent its proteolytic degradation upon topical application to chronic wounds. Phase diagrams were constructed based on turbidity as a function of GA to SA ratio and pH. Various GA-SA mixtures were compared for polydispersity index, zeta potential, Z-average, and ATR-FTIR spectra. Trypsin digestion and human dermal fibroblast scratch wound assay were done to evaluate the effects of EGF encapsulation. The onset pH values for coacervation and precipitation were closer together in high molecular weight GA (HWGA)-SA reaction mixtures than in low molecular weight GA (LWGA)-SA, which was attributed to strong H-bonding interactions between HWGA and SA probed by ATR-FTIR. EGF incorporation in both HWGA-SA precipitates and LWGA-SA coacervates below the isoelectric point of EGF, but not above it, suggests the contribution of electrostatic interactions between EGF and SA. EGF encapsulated in LWGA-SA coacervates was effectively protected from trypsin digestion and showed better in vitro scratch wound activity compared to free EGF. LWGA-SA coacervates are suggested as a novel delivery system for topical application of EGF to chronic wounds.
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ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics11100530