Spectroscopy studies of sandwich-type complex of silver(I) co-ordinated to nuclear fast red and adenine and its analytical applications

• Published in: Chemical papers Vol. 69; no. 4; pp. 510 - 517
• Main Authors: Cui, Yi, Gong, Yi-Jun, Su, Li, Wu, Cheng-Ke, Pang, Sheng-Bin, Feng, Su-Ling
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.04.2015; De Gruyter Open
• Subjects: adenine; Ag; Biochemistry; Biotechnology; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; fluorescence quenching; Industrial Chemistry/Chemical Engineering; Materials Science; Medicinal Chemistry; nuclear fast red; Original Paper; sandwich-type complex; nuclear fast red; sandwich-type complex; Ag; adenine; fluorescence quenching
• ISSN: 0366-6352; 1336-9075
• DOI: 10.1515/chempap-2015-0056

A novel fluorometric method for the determination of adenine was proposed, based on the fluorescence quenching of a nuclear fast red-silver complex as a small molecule optical probe in the presence of adenine. The characteristic spectra of absorption, resonance Rayleigh scattering (RRS) and the fluorescence of the system were analysed. Adenine, Ag + and nuclear fast red formed a sandwich-type complex. The composition of the compound and the mechanisms of fluorescence quenching were discussed. The optimal conditions for the reaction and the effects of co-existing substances were also investigated. The quenched fluorescence intensity was proportional to the concentration of adenine in the range of 0.03–0.90 µg mL −1 , with the limit of detection of 0.023 µg mL −1 . The method was applied satisfactorily to the determination of adenine in synthetic samples, herring sperm DNA, human sera and vitamin B 4 tablets with recoveries from 95.3 % to 105 %.