Isolation of polymorphic DNA segments from human chromosome 21

• Published in: Nucleic acids research Vol. 13; no. 17; pp. 6075 - 6088
• Main Authors: WatKins, Paul C., Tanzi, Rudolph E., Gibbons, Kerin T., Tricoli, James V., Landes, Greg, Eddy, Roger, Shows, Thomas B., Gusella, James F.
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 11.09.1985
• Subjects: Animals; Base Sequence; Biological and medical sciences; Cell Line; Chromatin. Chromosome; Chromosomes, Human, 21-22 and Y; Cloning, Molecular; DNA - isolation & purification; DNA, Recombinant - isolation & purification; Female; Fundamental and applied biological sciences. Psychology; Humans; Hybrid Cells - cytology; Male; Mice; Molecular and cellular biology; Molecular genetics; Nucleic Acid Hybridization; Pedigree; Polymorphism, Genetic; Repetitive Sequences, Nucleic Acid; Virus; Human; Hybrid cell; Cell line; DNA; Bactériophage; Somatic cell; Isolation; Molecular cloning; Polymorphism
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/13.17.6075

A somatic cell hybrid line containing only human chromosome 21 on a mouse background has been used as the source of DNA for construction of a recombinant phage library. Individual phages containing human inserts have been identified. Repeat-free human DNA subclones have been prepared and used to screen for restriction fragment length polymorphisms to provide genetic markers on chromosome 21. Nine independently isolated clones used as probes identified a total of 11 new RFLPs. Four of the DNA probes recovered from the library have been mapped unequivocally to chromosome 21 using a panel of somatic cell hybrid lines. A fifth probe detected an RFLP on chromosome 21 as well as sequences on other chromosomes. This set of RFLPs may now form the basis for construction of a genetic linkage map of human chromosome 21.