Genome-scale transcriptional analysis reveals key genes associated with the development of type II diabetes in mice

• Published in: Experimental and therapeutic medicine Vol. 13; no. 3; pp. 1044 - 1150
• Main Authors: Zhang, Yuchi, Han, Dongwei, Yu, Pengyang, Huang, Qijing, Ge, Pengling
• Format: Journal Article
• Language: English
• Published: Greece Spandidos Publications 01.03.2017; Spandidos Publications UK Ltd; D.A. Spandidos
• Subjects: Biomedical research; Cell cycle; Deoxyribonucleic acid; Diabetes; DNA; DNA methylation; Gene expression; Genomes; Glucose; Hyperglycemia; Insulin; Kinases; Metabolism; Obesity; Polymerase chain reaction; Proteins; Rodents; Signal transduction; Software; Studies; Type 2 diabetes; United States > US; China; microarray; mRNA; type II diabetes; gene expression
• ISSN: 1792-0981; 1792-1015
• DOI: 10.3892/etm.2017.4042

Diabetes mellitus is one of the primary diseases that pose a threat to human health. The focus of the present study is type II diabetes (T2D), which is caused by obesity and is the most prevalent type of diabetes. However, genome-scale transcriptional analysis of diabetic liver in the development process of T2D is yet to be further elucidated. Microassays were performed on liver tissue samples from three-, six- and nine-week-old mice with diabetes and mice to investigate differentially expressed mRNA. Based on the results of genome-scale transcriptional analysis, five genes were screened in the present study: chromobox 8 ( ), de-etiolated homolog 1 and damage specific DNA binding protein 1 associated 1 ( ), Phosphoinositide-3-kinase regulatory subunit 6 ( ), WD repeat domain 41 ( ) and Glycine Amidinotransferase ( ). At three weeks of age, no significant differences in levels or ratios of expression were observed. However, at six and nine weeks, expression of , , and was significantly upregulated (P<0.05) in the model group compared with the control group, whereas expression was significantly downregulated (P<0.05). These results suggest that T2D-related differential expression of genes becomes more marked with age, which was confirmed via reverse transcription-quantitative polymerase chain reaction. Genome-scale transcriptional analysis in diabetic mice provided a novel insight into the molecular. events associated with the role of mRNAs in T2D development, with specific emphasis upon , , , and . The results of the present study may provide rationale for the investigation of the target genes of these mRNAs in future studies.