Sulfobetaine methacrylate-functionalized graphene oxide-IR780 nanohybrids aimed at improving breast cancer phototherapy

• Published in: RSC advances Vol. 1; no. 63; pp. 38621 - 3863
• Main Authors: Leitão, Miguel M, Alves, Cátia G, de Melo-Diogo, Duarte, Lima-Sousa, Rita, Moreira, André F, Correia, Ilídio J
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 20.10.2020; The Royal Society of Chemistry
• Subjects: Absorption; Ammonium hydroxide; Biocompatibility; Breast cancer; Charge distribution; Chemical reduction; Chemistry; Colloids; Graphene; Hydrazines; Nanomaterials; Near infrared radiation; Polyethylene glycol; Size distribution; Surface charge; Surface chemistry
• ISSN: 2046-2069; 2046-2069
• DOI: 10.1039/d0ra07508f

The application of Graphene Oxide (GO) in cancer photothermal therapy is hindered by its lack of colloidal stability in biologically relevant media and modest Near Infrared (NIR) absorption. In this regard, the colloidal stability of GO has been improved by functionalizing its surface with poly(ethylene glycol) (PEG), which may not be optimal due to the recent reports on PEG immunogenicity. On the other hand, the chemical reduction of GO using hydrazine hydrate has been applied to enhance its photothermal capacity, despite decreasing its cytocompatibility. In this work GO was functionalized with an amphiphilic polymer containing [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide (SBMA) brushes and was loaded with IR780, for the first time, aiming to improve its colloidal stability and phototherapeutic capacity. The attained results revealed that the SBMA-functionalized GO displays a suitable size distribution, neutral surface charge and adequate cytocompatibility. Furthermore, the SBMA-functionalized GO exhibited an improved colloidal stability in biologically relevant media, while its non-SBMA functionalized equivalent promptly precipitated under the same conditions. By loading IR780 into the SBMA-functionalized GO, its NIR absorption increased by 2.7-fold, leading to a 1.2 times higher photothermal heating. In in vitro cell studies, the combination of SBMA-functionalized GO with NIR light only reduced breast cancer cells' viability to 73%. In stark contrast, by combining IR780 loaded SBMA-functionalized GO and NIR radiation, the cancer cells' viability decreased to 20%, hence confirming the potential of this nanomaterial for cancer photothermal therapy. IR780 loaded SBMA-coated GO displayed an improved colloidal stability in biologically relevant media and an enhanced photothermal capacity.