Characterization of KIAA0513, a novel signaling molecule that interacts with modulators of neuroplasticity, apoptosis, and the cytoskeleton

• Published in: Brain research Vol. 1121; no. 1; pp. 1 - 11
• Main Authors: Lauriat, Tara L., Dracheva, Stella, Kremerskothen, Joachim, Duning, Kerstin, Haroutunian, Vahram, Buxbaum, Joseph D., Hyde, Thomas M., Kleinman, Joel E., Alison McInnes, L.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 22.11.2006; Amsterdam Elsevier; New York, NY
• Subjects: Adult and adolescent clinical studies; Animals; Autopsy; Biological and medical sciences; Brain - physiology; Brain - physiopathology; Cerebellum; Cerebellum - metabolism; Cerebellum - pathology; DNA Primers; Female; Gene expression; Humans; In Situ Hybridization; KIBRA; Medical sciences; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - physiology; Nuclear Proteins - genetics; Nuclear Proteins - physiology; Oligonucleotide Array Sequence Analysis; Prefrontal Cortex - metabolism; Prefrontal Cortex - pathology; Psychology. Psychoanalysis. Psychiatry; Psychopathology. Psychiatry; Psychoses; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; RNA - genetics; RNA - isolation & purification; RNA, Messenger - genetics; RT-PCR; Schizophrenia; Schizophrenia - genetics; Signal Transduction; Yeast two-hybrid; Cerebellum; DSM-IV; TRADD; WWC1; HRP; Schizophrenia; kDa; EGFP; ACTB; KIBRA; ANCOVA; BSA; DMEM; FITC; RT-PCR; NMDA; FADD; DISC1; B2M; DENN; PBS; SDS; DLPFC; N-terminal; Yeast two-hybrid; IP; DAPI; GST; PKC; Gene expression; PMI; NBT/BCIP; HAX-1; MADD; INTS4; GAPDH; MAP; MTE; Human; Central nervous system; Encephalon; Psychosis; Characterization; Polymerase chain reaction; Signal transduction; Cell death; Plasticity; Cytoskeleton; Apoptosis
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2006.08.099

KIAA0513 was previously identified as upregulated in the dorsolateral prefrontal cortex of subjects with schizophrenia by microarray analysis. In the present study, the differential expression in the schizophrenic subjects was confirmed by quantitative RT-PCR. The limited homology to proteins of known function and lack of functional domains in the encoded protein have made it difficult to predict a function for KIAA0513. We used in situ hybridization, RNA blots, western blots, and immunocytochemistry to examine KIAA0513 expression in normal brain and peripheral tissues. The gene is ubiquitously expressed but is enriched in the brain, particularly in the cerebellum. Finally, interacting proteins were identified using a yeast two-hybrid screen to functionally characterize the protein. KIAA0513 interacts with KIBRA, HAX-1, and INTS4, which also interact with proteins involved in neuroplasticity, apoptosis, and cytoskeletal regulation. Therefore, KIAA0513 is likely to be involved in signaling pathways related to these processes.