Enhanced production of Scenedesmus spp. (green microalgae) using a new medium containing fermented swine wastewater

• Published in: Bioresource technology Vol. 98; no. 11; pp. 2220 - 2228
• Main Authors: Kim, M.K., Park, J.W., Park, C.S., Kim, S.J., Jeune, K.H., Chang, M.U., Acreman, J.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.08.2007; Elsevier Science
• Subjects: Amino acids; animal manures; Animal productions; Animals; Applied sciences; Batch culture; Biological and medical sciences; Biotechnology; Cell Culture Techniques; cell division; chemical composition; Chloroplasts - ultrastructure; Culture Media; Exact sciences and technology; excretion; Fermentation; Fermented swine urine; Fundamental and applied biological sciences. Psychology; Long-term culture; Methods. Procedures. Technologies; microalgae; Microbial engineering. Fermentation and microbial culture technology; photosynthesis; Pollution; Production; Pyrenoid; Scenedesmus; Scenedesmus - growth & development; Scenedesmus - metabolism; Scenedesmus - ultrastructure; Scenedesmus acutus; Scenedesmus quadricauda; Scenedesmus spinosus; secondary metabolites; swine; Swine - urine; Terrestrial animal productions; urine; Vertebrates; wastewater; Wastewaters; Water Purification; Water treatment and pollution; Scenedesmus; Batch culture; Long-term culture; Pyrenoid; Fermented swine urine; Production; Urine; Algae; Chlorophyceae; Fermentation; Medium containing; Long term; Pig barn; Waste water; Vertebrata; Mammalia; Chlorophyta; Swine; Batch process; Artiodactyla; Microorganism; Ungulata; Thallophyta
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2006.08.031

Fermented swine urine (3%) (v/v) was added to a control medium (CT), named KEP I, and an aquatic microalgal culture (10% Bold’s Basal Medium) for growing mixed Scenedesmus species. During a two-month period, the KEP I medium effected, the delayed onset of the stationary phase of cell division in a batch culture. After 31 days, of culturing, the growth rate (3-fold), dry weight (2.6-fold) and amino acid levels (2.7-fold), and secondary metabolites including chlorophyll a (2.1-fold), astaxanthin (2.8-fold), lutein (2.7-fold) and α (greater than 30-fold) and β-carotene (greater than 5-fold) increased a greater degree in Scenedesmus grown in KEP I medium than in CT medium. Total lipids were much less in cells grown in KEP I than those grown in CT. An increased quantum yield of photosystem II of the aquatic microalgae. The KEP I medium should improve the cost efficiency of industrial mass batch cultures for CO 2 sequestration, bioremediation, phytonutrients, agricultural fertilizers, and microalgal stock for the species preservation of aquaculture strains for use in young fish feed. It may also serve to attenuate negative environmental impact via the recycling of animal wastewater.