pDblet, a stable autonomously replicating shuttle vector for Schizosaccharomyces pombe

We have constructed a new multipurpose stable shuttle vector for the fission yeast Schizosaccharomyces pombe (Sp). Plasmid pDblet was designed to provide convenient features for molecular work and to overcome the inconveniences of previously designed Sp vectors. It contains the Sp ura4 gene as selec...

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Bibliographic Details
Published inGene Vol. 164; no. 1; pp. 173 - 177
Main Authors Brun, Christine, Dubey, Dharani D., Huberman, Joel A.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.01.1995
Elsevier
Subjects
ARS
autonomously replicating sequence
Biochemistry, Molecular Biology
Bioinformatics
cloning
Computer Science
DNA Replication
fission yeast
gene transfer
Genetic Markers
genetic transformation
Genetic Vectors
Life Sciences
Mitosis
Molecular biology
plasmid
plasmid vectors
Replication Origin
reporter genes
Saccharomycetales
Schizosaccharomyces - genetics
Schizosaccharomyces pombe
Selection, Genetic
Transformation, Genetic
ura4 gene
ARS
E
nt
ura4
ori
plasmid
bp
Ap
Sc
R
ARS (ars)
kb
fission yeast
replication origin
MCS
Sp
cloning
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Summary:We have constructed a new multipurpose stable shuttle vector for the fission yeast Schizosaccharomyces pombe (Sp). Plasmid pDblet was designed to provide convenient features for molecular work and to overcome the inconveniences of previously designed Sp vectors. It contains the Sp ura4 gene as selectable marker and a new highly efficient ARS (autonomously replicating sequence) element, allowing the vector to remain stable as a monomer in Sp. In addition, pDblet transforms Sp with high efficiency and has high mitotic stability and low copy number.
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ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(95)00497-T