Genome-Wide Identification and Expression Profiles of C-Repeat Binding Factor Transcription Factors in Betula platyphylla under Abiotic Stress

• Published in: International journal of molecular sciences Vol. 24; no. 13; p. 10573
• Main Authors: Zhang, Xiang, Yu, Jiajie, Wang, Ruiqi, Liu, Wenxuan, Chen, Song, Wang, Yiran, Yu, Yue, Qu, Guanzheng, Chen, Su
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24.06.2023; MDPI
• Subjects: ABA; Abiotic stress; Abscisic acid; Arabidopsis; Betula platyphylla; Binding; bioinformatic analysis; CBF protein; CBF transcription factor family; Chromosomes; Cold; Cold treatment; Collinearity; Environmental stress; Gene expression; Genes; Genomes; Phylogenetics; Physicochemical properties; Protein structure; Proteins; salt treatment; Transcription factors; Transgenic plants; Trees; ABA; transcription activating activity; Betula platyphylla; CBF transcription factor family; bioinformatic analysis; cold treatment; salt treatment
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms241310573

CBF (C-repeat binding factor) transcription factor subfamily belongs to AP2/ERF (Apetala 2/ethylene-responsive factor) transcription factor family, known for playing a vital role in plant abiotic stress response. Although some CBF transcription factors have been identified in several species, such as , tobacco, tomato and poplar, research of CBF focus mainly on model plant and have not been reported in yet. In this study, a total of 20 BpCBF subfamily members were identified. The conserved domains, physicochemical properties, exon-intron gene structure and the structure of conserved protein motifs of BpCBFs were analyzed via bioinformatic tools. The collinearity analysis of CBF genes was performed between and , and . The -acting elements in the promoter region of s were identified, which were mainly environmental stress-related and hormone-related element components. In this case, the expression patterns of the 20 s upon ABA or salt treatment were investigated. Most of these transcription factors were responsive to ABA or salt stress in different plant tissues. The up-regulation trend upon cold treatment of the six cold-responsive genes validated by qRT-PCR was consistent with the result of RNA-seq. BpCBF7 showed transcription activating activity. This study sheds light on the responses of BpCBFs to abiotic stress and provides a reference for further study of CBF transcription factors in woody plants.