PCR–RFLP of the mitochondrial cytochrome oxidase (subunit I) gene provides diagnostic markers for selected Diabrotica species (Coleoptera: Chrysomelidae)

Adult and larval identification of Diabrotica can be difficult. Some adult identifications require considerable taxonomic experience while larvae of many Diabroticaspecies are morphologically indistinguishable. This study was conducted to determine whether 12 pest and non-pest Diabrotica species cou...

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Bibliographic Details
Published inBulletin of entomological research Vol. 91; no. 6; pp. 419 - 427
Main Authors Clark, T.L., Meinke, L.J., Foster, J.E.
Format Journal Article
LanguageEnglish
Published Cambridge, UK Cambridge University Press 01.12.2001
Subjects
Adults
Animals
Base Sequence
Biological and medical sciences
Coleoptera - enzymology
Coleoptera - genetics
Corn
Cytochrome
DNA
Electron Transport Complex IV - genetics
Entomology
Enzymes
Fundamental and applied biological sciences. Psychology
General aspects and techniques. Study of several systematic groups. Models
Generalities
Genes, Insect
Genetic Markers
Invertebrates
Larvae
Miscellaneous techniques
Mitochondria - enzymology
Molecular Sequence Data
Pests
Phytopathology. Animal pests. Plant and forest protection
Polymerase Chain Reaction - methods
Polymorphism
Polymorphism, Restriction Fragment Length
Protozoa. Invertebrates
Techniques
United States > US
Chrysomelidae
Coleoptera
Enzyme
Insecta
Cytochrome-c oxidase
Molecular marker
Method
Pest
Gene
Diabrotica
Arthropoda
Specific identification
Oxidoreductases
Invertebrata
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Summary:Adult and larval identification of Diabrotica can be difficult. Some adult identifications require considerable taxonomic experience while larvae of many Diabroticaspecies are morphologically indistinguishable. This study was conducted to determine whether 12 pest and non-pest Diabrotica species could be separated using polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP). A 1308 bp portion of the mitochondrial cytochrome oxidase subunit I gene (COI) was amplified using PCR and digested using several restriction endonucleases. Double digests of COI amplicons with AluI and MspI resolved on polyacrylamide gels revealed several diagnostic inter- and intraspecific polymorphisms. A key to the 12 species was constructed using the PCR–RFLP patterns.
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ISSN:0007-4853
1475-2670
DOI:10.1079/BER2001130