Therapeutic effects of hyperbaric oxygen in a rat model of endothelin-1-induced focal cerebral ischemia

• Published in: Brain research Vol. 1153; pp. 204 - 213
• Main Authors: Huang, Zhen-Xing, Kang, Zhi-Min, Gu, Guo-Jun, Peng, Guang-Neng, Yun, Liu, Tao, Heng-Yi, Xu, Wei-Gang, Sun, Xue-Jun, Zhang, John H
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 11.06.2007; Amsterdam Elsevier; New York, NY
• Subjects: Analysis of Variance; Animals; Bax; Bcl-2; bcl-2-Associated X Protein - metabolism; Biological and medical sciences; Brain Edema - etiology; Brain Edema - therapy; Brain Infarction - etiology; Brain Infarction - therapy; Brain Ischemia - chemically induced; Brain Ischemia - complications; Brain Ischemia - therapy; Disease Models, Animal; Endothelin-1; Focal cerebral ischemia; Gene Expression Regulation - drug effects; Hyperbaric oxygen; Hyperbaric Oxygenation - methods; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism; Hypoxia-inducible factor-1α; Male; Medical sciences; Neurology; Proto-Oncogene Proteins c-bcl-2 - metabolism; Rats; Rats, Sprague-Dawley; Vascular diseases and vascular malformations of the nervous system; Bcl-2; PDVF; HIF-1α; hyperbaric oxygen; phosphate-buffered saline; analysis of variance; HBO; ANOVA; endothelin-1; polyvinylidene; MCAO; PBS; Bcl-2/adenovirus E1B 19-kDa protein-interacting protein 3; BNIP3; Focal cerebral ischemia; MCA; ATA; middle cerebral artery; ET; endothelin; atmosphere absolute; ET-1; hypoxia-inducible factor-1α; Bax; middle cerebral artery occlusion; Hyperbaric oxygen; Hypoxia-inducible factor-1α; Endothelin-1; Animal model; Rat; Rodentia; Central nervous system; Cardiovascular disease; Endothelin 1; Encephalon; Vertebrata; Mammalia; Treatment; Ischemia; Animal; Hyperbaric oxygen;Hypoxia-inducible factor-la;Bcl-2;Bax;Endothelin-1;Focal cerebral ischemia
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2007.03.061

Abstract It has been established that hyperbaric oxygen (HBO) treatment reduces brain edema, decreases infarct volume, contributes to neurological functional recovery and suppresses apoptosis in suture-induced focal cerebral ischemic animal models. In the present study, we evaluated the therapeutic effect of HBO in an endothelin-1-induced focal cerebral ischemia in rats and explored the associated mechanisms of HBO-induced brain protection. One hundred twenty male Sprague–Dawley rats (280 to 320 g) were randomly assigned to sham, focal cerebral ischemia and focal cerebral ischemia treated with HBO groups. Brain water content, neurological function, morphology and molecular biological markers were assessed. HBO (100% O2 , 2.5 atmosphere absolute for 2 h) was initiated at 1 h after focal cerebral ischemia. Rats were killed at 24 h to harvest tissues for Western blot or for histology. In HBO-treated animals, an enhanced ratio of Bcl-2 and Bax and a reduced expression of hypoxia-inducible factor-1α (HIF-1α) in the hippocampus after focal cerebral ischemia were observed. These results indicate that HBO provides brain protection that is probably associated with the inhibition of HIF-1α and the elevation of Bcl-2.