Defective callose walls and cell plates during abnormal meiosis cause male-sterility in the oat mutant zbs1

• Published in: Journal of Integrative Agriculture Vol. 15; no. 2; pp. 241 - 248
• Main Author: SHI Xiao, WU Jin-xia, ZHOU Hai-tao, YANG Xiao-hong, LI Tian-liang, ZHANG Xin-jun, YANG Cai, HAN Xiao (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences/National Key Facility for Gene Resources and Gene Improvement, Beijing 100081, P.R.China Zhangjiakou Academy of Agricultural Sciences, Zhangjiakou 075000, P.R.China)
• Format: Journal Article
• Language: English
• Published: Science Press 01.02.2016; Biotechnology Research Institute,Chinese Academy of Agricultural Sciences/National Key Facility for Gene Resources and Gene Improvement,Beijing 100081,P.R.China%Zhangjiakou Academy of Agricultural Sciences,Zhangjiakou 075000,P.R.China; KeAi Communications Co., Ltd
• Subjects: Avena nuda; callose; callose male sterility meiosis microspore naked oat; cell walls; chromosomes; epitopes; germination; inflorescences; male fertility; male sterility; meiosis; microspore; microspores; mutants; naked oat; oats; pollen; propidium; scanning electron microscopy; staining; 减数分裂异常; 板; 电子显微镜观察; 细胞壁; 缺陷; 胼胝质; 裸燕麦; 雄性不育突变体; callose; meiosis; microspore; naked oat; male sterility
• ISSN: 2095-3119; 2352-3425
• DOI: 10.1016/S2095-3119(15)61086-3

During meiosis in flowering plants,degradation of the callose wall in tetrads releases newly produced microspores,which develop into mature pollen grains.In this study,we identified zbs1,a male-sterile mutant of naked oat（Avena nuda L.）that displayed complete spikelet sterility due to inviable mature pollen.The abnormal pollen grains originated from microspores with a defective callose wall and cell plate during meiosis.The defective callose wall and cell plate of the zbs1 mutant were detected by the labeling of cell wall epitopes（β-1,3-glucan） with immunogold during meiosis,and an abnormal chromosome configuration was observed by propidium iodide staining.The mature pollen grains of the zbs1 mutant were irregular in shape,and abnormal germination was observed by scanning electron microscopy.Together,our results indicate that the cause of male sterility in zbs1 is abnormal meiosis.