Si-doping increases the adjuvant activity of hydroxyapatite nanorods

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 174; pp. 300 - 307
• Main Authors: Wang, Xiupeng, Ihara, Shu, Li, Xia, Ito, Atsuo, Sogo, Yu, Watanabe, Yohei, Tsuji, Noriko M., Yamazaki, Atsushi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2019
• Subjects: Adjuvant; Cytokine secretion; Hydroxyapatite; Si-doping; Th1; Th2; Hydroxyapatite; Adjuvant; Cytokine secretion; Th1; Si-doping; Th2
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2018.11.026

Si-doping in HA nanorods increases adjuvant activity, promotes maturation and cytokine secretion of BMDCs, increases both Th1 and Th2 cytokines secretion. [Display omitted] •Si-doping in HA nanorods increases adjuvant activity.•Si-doping in HA nanorods promotes maturation and cytokine secretion of BMDCs.•Si-doping in HA increases both Th1 and Th2 cytokines secretion.•Si-doped HA accelerates antigen delivery in lymph node in vivo. Recombinant protein-based vaccines generally show limited immunogenicity and need adjuvants to achieve robust immune responses. Herein, to combine the excellent biocompatibility of hydroxyapatite (HA) and exciting adjuvant activity of silica, Si-doped HA nanorods with Si/P molar ratio from 0 to 0.65 were hydrothermally synthesized and evaluated as immunoadjuvants. Si-doping decreases the size and increases the BET surface area of the nanorods. Si-doping in HA nanorods increases the in vitro adjuvant activity, including CD11c+CD86+ expression and cytokine secretion of bone marrow derived dendritic cells (BMDCs). Moreover, Si-doping in HA increases the ex vivo adjuvant activity as shown by the increase in both Th1 and Th2 cytokines secretion. Si-doped HA nanorods are promising as a new immunoadjuvant.