Si-doping increases the adjuvant activity of hydroxyapatite nanorods
Si-doping in HA nanorods increases adjuvant activity, promotes maturation and cytokine secretion of BMDCs, increases both Th1 and Th2 cytokines secretion. [Display omitted] •Si-doping in HA nanorods increases adjuvant activity.•Si-doping in HA nanorods promotes maturation and cytokine secretion of B...
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Published in | Colloids and surfaces, B, Biointerfaces Vol. 174; pp. 300 - 307 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
01.02.2019
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Subjects | |
Online Access | Get full text |
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Summary: | Si-doping in HA nanorods increases adjuvant activity, promotes maturation and cytokine secretion of BMDCs, increases both Th1 and Th2 cytokines secretion.
[Display omitted]
•Si-doping in HA nanorods increases adjuvant activity.•Si-doping in HA nanorods promotes maturation and cytokine secretion of BMDCs.•Si-doping in HA increases both Th1 and Th2 cytokines secretion.•Si-doped HA accelerates antigen delivery in lymph node in vivo.
Recombinant protein-based vaccines generally show limited immunogenicity and need adjuvants to achieve robust immune responses. Herein, to combine the excellent biocompatibility of hydroxyapatite (HA) and exciting adjuvant activity of silica, Si-doped HA nanorods with Si/P molar ratio from 0 to 0.65 were hydrothermally synthesized and evaluated as immunoadjuvants. Si-doping decreases the size and increases the BET surface area of the nanorods. Si-doping in HA nanorods increases the in vitro adjuvant activity, including CD11c+CD86+ expression and cytokine secretion of bone marrow derived dendritic cells (BMDCs). Moreover, Si-doping in HA increases the ex vivo adjuvant activity as shown by the increase in both Th1 and Th2 cytokines secretion. Si-doped HA nanorods are promising as a new immunoadjuvant. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0927-7765 1873-4367 |
DOI: | 10.1016/j.colsurfb.2018.11.026 |