Anti-Inflammatory and Gut Microbiota Modulation Potentials of Flavonoids Extracted from Passiflora foetida Fruits

• Published in: Foods Vol. 12; no. 15; p. 2889
• Main Authors: Han, Xiangpeng, Song, Ya, Huang, Riming, Zhu, Minqian, Li, Meiying, Requena, Teresa, Wang, Hong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 29.07.2023; MDPI
• Subjects: 1-Phosphatidylinositol 3-kinase; AKT protein; anti-inflammatory activity; Anti-inflammatory agents; Antibodies; Bioactive compounds; Fatty acids; Feces; flavonoid-rich fraction; Flavonoids; Food science; Fruits; gut microbiota; Immune system; Inflammation; Inflammatory bowel disease; Interleukin 6; Interleukins; Intestinal microflora; MAP kinase; Metabolites; Microbiota; Microorganisms; Microscopy; Modulation; NF-κB protein; Nitric oxide; Passiflora foetida; Passiflora foetida fruits; Penicillin; Phosphorylation; Polyphenols; Proteins; short-chain fatty acids; Tumor necrosis factor-α; United States > US; China; Passiflora foetida fruits; flavonoid-rich fraction; short-chain fatty acids; gut microbiota; anti-inflammatory activity
• ISSN: 2304-8158; 2304-8158
• DOI: 10.3390/foods12152889

This study aimed to explore the anti-inflammatory and gut microbiota modulation potentials of flavonoid-rich fraction (PFF) extracted from fruits. The results showed that PFF markedly reduced the production of nitric oxide (NO), tumor necrosis factor α (TNF-α), and interleukin 6 (IL-6) in LPS-stimulated RAW 264.7 cells. Meanwhile, PFF treatment also effectively decreased the phosphorylation levels of MAPK, PI3K/Akt, and NF-κB signaling-pathway-related proteins (ERK, JNK, p38, Akt, and p65). Moreover, PFF had an impact on microbial composition and metabolites in a four-stage dynamic simulator of human gut microbiota (BFBL gut model). Specifically, PFF exhibited the growth-promoting ability of several beneficial bacteria, including , , , and , and short-chain fatty acid (SCFA) generation ability in gut microbiota. In addition, spectroscopic data revealed that PFF mainly contained five flavonoid compounds, which may be bioactive compounds with anti-inflammatory and gut microbiota modulation potentials. Therefore, PFF could be utilized as a natural anti-inflammatory agent or supplement to health products.