High Genetic Diversity and Virulence Potential in Bacillus cereus sensu lato Isolated from Milk and Cheeses in Apulia Region, Southern Italy

The group includes species that act as food-borne pathogens causing diarrheal and emetic symptoms. They are widely distributed and can be found in various foods. In this study, out of 550 samples of milk and cheeses, 139 (25.3%) were found to be contaminated by ( ). One isolate per positive sample w...

Full description

Saved in:
Bibliographic Details
Published inFoods Vol. 12; no. 7; p. 1548
Main Authors Bianco, Angelica, Normanno, Giovanni, Capozzi, Loredana, Del Sambro, Laura, Di Fato, Laura, Miccolupo, Angela, Di Taranto, Pietro, Caruso, Marta, Petruzzi, Fiorenza, Ali, Ashraf, Parisi, Antonio
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 06.04.2023
MDPI
Subjects
B. cereus
Bacillus cereus
Cheese
Dairy products
Diarrhea
Food
Food consumption
Food contamination
Food contamination & poisoning
food safety
Food science
Genes
Genetic diversity
Health risks
Manufacturers
Milk
MLST
Multilocus sequence typing
Public health
Statistical analysis
Virulence
Italy
Belgium
United States > US
China
food safety
B. cereus
MLST
dairy products
Online AccessGet full text

Cover

More Information
Summary:The group includes species that act as food-borne pathogens causing diarrheal and emetic symptoms. They are widely distributed and can be found in various foods. In this study, out of 550 samples of milk and cheeses, 139 (25.3%) were found to be contaminated by ( ). One isolate per positive sample was characterized by Multilocus Sequence Typing (MLST) and for the presence of ten virulence genes. Based on MLST, all isolates were classified into 73 different sequence types (STs), of which 12 isolates were assigned to new STs. Virulence genes detection revealed that 90% and 61% of the isolates harboured the and the gene cluster, respectively. Ninety-four percent of the isolates harboured the enterotoxin genes and ; 8% of the isolates possessed the gene. Thirty-eight different genetic profiles were identified, suggesting a high genetic diversity. Our study clearly shows the widespread diffusion of potentially toxigenic isolates of in milk and cheeses in the Apulia region highlighting the need to adopt GMP and HACCP procedures along every step of the milk and cheese production chain in order to reduce the public health risk linked to the consumption of foods contaminated by
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2304-8158
2304-8158
DOI:10.3390/foods12071548