Neuropeptide Y receptor in the rat brain

• Published in: European journal of biochemistry Vol. 145; no. 3; pp. 525 - 530
• Main Authors: UNDÉN, Anders, TATEMOTO, Kazuhiko, MUTT, Viktor, BARTFAI, Tamas
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.01.1984; Blackwell
• Subjects: Aminoacids, peptides. Hormones. Neuropeptides; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Brain - metabolism; cortex; Fundamental and applied biological sciences. Psychology; Hydrogen-Ion Concentration; Kinetics; Male; Nerve Tissue Proteins - metabolism; Neuropeptide Y; Oxidation-Reduction - drug effects; Protein Denaturation; Proteins; Rats; Rats, Inbred Strains; Receptors, Cell Surface - metabolism; Receptors, Neuropeptide Y; Subcellular Fractions - metabolism; Sulfhydryl Reagents; Temperature; Cerebral cortex; Vertebrata; Mammalia; Rat; Animal; Rodentia; Brain(Invertebrata); Neurotransmitter; Molecular interaction; Neuropeptide; Biological receptor
• ISSN: 0014-2956; 1432-1033
• DOI: 10.1111/j.1432-1033.1984.tb08588.x

The specific binding of the chloramine‐T iodinated neuropeptide Y (125I‐NPY) to membranes from rat cerebral cortex was investigated using equilibrium binding and kinetic methods. The equilibrium binding of 125I‐NPY at 37°C was characterized by a Kd value of 0.38 nM. The receptor densities in the cerebral cortex, hypothalamus and cerebellum were 0.45 pmol/mg, 0.47 pmol/mg and 0.04 pmol/mg protein respectively. The binding site for 125I‐NPY was sensitive to treatment with proteolytic enzymes and thiol reagents. The binding showed a sharp optimum at pH 7 ‐ 7.7 and was inhibited by increasing concentrations of Mg2+