N‐linked glycopeptides with blood group determinants lacking neuraminic acid from the epithelial cells of rat small and large intestine

• Published in: European journal of biochemistry Vol. 203; no. 1‐2; pp. 285 - 293
• Main Authors: NORRSELL, Henrik, BENGTSSON, Jonas, JOVALL, Per‐Åke, HANSSON, Gunnar C.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.01.1992; Blackwell
• Subjects: Animals; Biological and medical sciences; Blood Group Antigens - immunology; Chromatography, Gel; Fundamental and applied biological sciences. Psychology; Fundamental immunology; glycans; Glycopeptides - immunology; Glycosphingolipids - immunology; Humans; Immunohematology; Intestinal Mucosa - cytology; Intestinal Mucosa - metabolism; Intestine, Large - cytology; Intestine, Large - metabolism; Intestine, Small - cytology; Intestine, Small - metabolism; Leukocyte immunology; Magnetic Resonance Spectroscopy; Mass Spectrometry; Neuraminic Acids - metabolism; Rats; Rat; Rodentia; Gut; NMR spectrometry; Glycan; Blood; Vertebrata; Blood group; Mammalia; Glycopeptide; Neuraminic acid; Epithelial cell; Mass spectrometry
• ISSN: 0014-2956; 1432-1033
• DOI: 10.1111/j.1432-1033.1992.tb19858.x

The N‐linked type of glycans were prepared as their glycopeptides after pronase digestion of the epithelial cells from the small and large intestine of two inbred strains of rat. These glycopeptides were analysed for sugar composition, for blood‐group activity, by 1H‐NMR spectroscopy, and after permethylation by electron‐impact mass spectrometry. The glycopeptides were of the triantennary and tetraantennary types with intersected GlcNAc. The terminal parts were, in contrast to most N‐linked glycans, devoid of neuraminic acid residues. Instead they contained blood‐group determinants. Blood‐group‐H types 1 (Fucα1 – 2Galβ1 – 3GlcNAc) and 2 (Fucα1 – 2Galβ1 – 4GlcNAc) were found in the small and large intestines of both strains, although type‐1 predominated. One rat strain (GOT‐W) did not express blood‐group‐A glycopeptides in the small intestine, but the large intestine from the same strain did. The other strain (GOT‐BW) expressed blood‐group‐A determinants in the small intestine. The lack of neuraminic acid residues in the small and large intestine and of blood‐group‐B activity in the large intestine differed from that found in glycosphingolipids obtained from the same organs.