Cyclic assembly-disassembly of cortical microtubules during maturation and early development of starfish oocytes

• Published in: Developmental biology Vol. 103; no. 2; pp. 493 - 503
• Main Authors: Schroeder, Thomas E., Otto, Joann J.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.01.1984; Elsevier
• Subjects: Adenine - analogs & derivatives; Adenine - pharmacology; Animals; Biological and medical sciences; Cell Nucleus - ultrastructure; Early stages. Segmentation. Gastrulation. Neurulation; Embryology: invertebrates and vertebrates. Teratology; Female; Fertilization; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Kinetics; Marine; Meiosis; Microtubules - drug effects; Microtubules - physiology; Microtubules - ultrastructure; Oocytes - growth & development; Oocytes - ultrastructure; Pisaster ochraceus; Starfish; Asteroidea; Hormone; Marine environment; Echinodermata; Cell cycle; Cytoskeleton; Microtubule; Invertebrata; Fecundation; Immunofluorescence; Oocyte; Oocyte maturation
• ISSN: 0012-1606; 1095-564X
• DOI: 10.1016/0012-1606(84)90336-1

An extensive array of cortical microtubules in oocytes of the starfish Pisaster ochraceus undergoes multiple cycles of disappearance and reapparance during maturation and early development. These events were studied in isolated fragments of the oocyte cortex stained with antitubulin antibodies for indirect immunofluorescence. The meshwork of long microtubules is present in the cortex (a) of immature oocytes, i.e., before treatment with the maturation-inducing hormone 1-methyladenine, (b) for 10–20 min after treatment with 1-methyladenine, (c) after formation of the second polar body (in reduced numbers in unfertilized oocytes), and (d) in the intermitotic period between first and second cleavage divisions. The array of cortical microtubules is absent in oocytes (a) undergoing germinal vesicle breakdown, (b) during the two meiotic divisions (polar body divisions), and (c) during mitosis of the first and, perhaps, subsequent cleavage divisions. The cycle of assembly-disassembly of cortical microtubules is synchronized to the cycle of nuclear envelope breakdown and reformation and to the mitotic cycle; specifically, cortical microtubules are present when a nucleus is intact (germinal vesicle, female pronucleus, zygote nucleus, blastomere nucleus) and are absent whenever a meiotic or mitotic spindle is present. These findings are discussed in terms of microtubule organizing centers in eggs, possible triggers for microtubule assembly and disassembly, the eccentric location of the germinal vesicle, and the regulation of oocyte maturation and cell division.