Extracellular biosynthesis of iron oxide nanoparticles by Bacillus subtilis strains isolated from rhizosphere soil

• Published in: Biotechnology and bioprocess engineering Vol. 17; no. 4; pp. 835 - 840
• Main Authors: Sundaram, P. Alagu, Augustine, Robin, Kannan, M.
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean Society for Biotechnology and Bioengineering 01.08.2012; Springer Nature B.V; 한국생물공학회
• Subjects: Analysis; Bacillus subtilis; Bacteria; Bioengineering; Biosynthesis; Biotechnology; Chemistry; Chemistry and Materials Science; E coli; Escherichia coli; Experiments; Fourier transform infrared spectroscopy; Grain size; Industrial and Production Engineering; Iron oxides; Nanoparticles; Research Paper; Rhizosphere; Scanning electron microscopy; soil; Spectrum analysis; Studies; Temperature; 생물공학; nanoparticles; organic capping; reduction; biosynthesis; Fe; O
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-011-0582-9

The biological synthesis of nanoparticles is emerging as a potential method for nanoparticle synthesis due to its non-toxicity and simplicity. We report the ability of Bacillus subtilis strains isolated from rhizosphere soil to produce iron oxide nanoparticles. B. subtilis strains having the potential for the extracellular biosynthesis of Fe 3 O 4 nanoparticles were isolated from rhizosphere soil, identified and characterized. A bactericidal protein subtilin was isolated from all the isolates of B. subtilis , which is a characteristic for the species. The isolated subtilin was tested against the bacterial strain, E. coli . The supernatant of the bacterial culture was used for the synthesis of Fe 3 O 4 nanoparticles. The formation of nanoparticles was assessed by using UV-Visible spectrophotometer. FTIR and SEM analysis were used in order to confirm the formation and size of the nanoparticles. Further, the effect of incubation time, pH, and temperature on the formation of Fe 3 O 4 nanoparticles was studied. The successful synthesis of stabilized Fe 3 O 4 nanoparticles, which was capped by the organic group, indicates the applicability of the isolated B. subtilis strain for the bulk synthesis of iron oxide nanoparticles.