Evidence for the existence of cytoskeleton-bound polysomes in plants

When conventional, high ionic strength buffers were used for the isolation of polysomes from pea plante, less than 20% were retained in the detergent-insoluble pellet. Reducing Tris, K + and Mg ++ to 10 mM increased retention to 70%, and when a new, microfilament-stabilizing buffer was used, retenti...

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Published inCell biology international reports Vol. 15; no. 10; pp. 973 - 981
Main Authors Davies, E., Fillingham, B.D., Oto, Y., Abe, S.
Format Journal Article
LanguageEnglish
Published London Elsevier B.V 01.10.1991
San Diego, CA Academic Press
New York, NY
Subjects
Biological and medical sciences
Cell physiology
Cytoskeleton - chemistry
Fabaceae - chemistry
Fundamental and applied biological sciences. Psychology
Magnesium
Molecular and cellular biology
Plants, Medicinal
Polyribosomes - chemistry
Potassium
Tromethamine
CBP
PTE
TBP
LP
PMSF
M + S
DOC
CSK
FP
MBP
SP
MSB
Cytoskeleton
Vegetals
Fixation
Polysome
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Summary:When conventional, high ionic strength buffers were used for the isolation of polysomes from pea plante, less than 20% were retained in the detergent-insoluble pellet. Reducing Tris, K + and Mg ++ to 10 mM increased retention to 70%, and when a new, microfilament-stabilizing buffer was used, retention increased to 80%. Conditions which favoured polysome pelleting at lower g forces permitted the retention of actin in the pellet. The data are consistent with the hypothesis that higher plants, like animals, contain cytoskeleton-(actin)-bound polysomes.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0309-1651
1878-240X
DOI:10.1016/0309-1651(91)90147-B