Cervical and Prostate Primary Epithelial Cells Are Not Productively Infected but Sequester Human Immunodeficiency Virus Type 1

• Published in: The Journal of infectious diseases Vol. 183; no. 8; pp. 1204 - 1213
• Main Authors: Dezzutti, Charlene S., Guenthner, Patricia C., Cummins, James E., Cabrera, Thania, Marshall, James H., Dillberger, Alexa, Lal, Renu B.
• Format: Journal Article
• Language: English
• Published: Chicago, IL The University of Chicago Press 15.04.2001; University of Chicago Press; Oxford University Press
• Subjects: Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Biological and medical sciences; Cathepsin D - pharmacology; CD4 Antigens - analysis; CD4-Positive T-Lymphocytes - virology; Cell Line; Cell lines; Cells, Cultured; Cervix Uteri - immunology; Cervix Uteri - virology; Cultured cells; Epithelial cells; Epithelial Cells - immunology; Epithelial Cells - virology; Female; Galactosylceramides - analysis; HIV; HIV 1; HIV-1 - isolation & purification; HIV-1 - physiology; HLA-DR Antigens - analysis; HT29 cells; Human immunodeficiency virus 1; Human viral diseases; Humans; Infections; Infectious diseases; Intestinal Mucosa - virology; Lymphocyte Activation; Lymphocytes - immunology; Lymphocytes - virology; Major Articles; Male; Medical sciences; Pharmacology. Drug treatments; Polymerase chain reaction; Prostate - immunology; Prostate - virology; Receptors; Receptors, CCR5 - analysis; Receptors, CXCR4 - analysis; Viral diseases; Viral diseases of the lymphoid tissue and the blood. Aids; Viruses; HIV-1 virus; Transmission; CC chemokine; Cell surface; Blood; Ceramide; Cervicitis; Infected cell; Urogenital system; Human; Immunopathology; Retroviridae; AIDS; Lentivirus; Immune deficiency; Female genital diseases; Infection; Virus; Blood cell; Cell line; Viral disease; Epithelial cell; Human immunodeficiency virus; Prostate; Uterine cervix diseases
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1086/319676

Primary prostate and cervical epithelial cells and epithelial cell lines were examined for human immunodeficiency virus type 1 (HIV-1) infection or transmission to peripheral blood mononuclear cells (PBMC). Neither cell-free nor cell-associated HIV-1 infected primary epithelial cells or cell lines. Pretreatment of HIV-1 to enhance CD4-independent entry did not augment infection. Cell surface expression was detected for galactosyl ceramide but not for CC-chemokine receptor 5, CXC-chemokine receptor 4, or CD4. The ability to transfer HIV-1 to resting or activated PBMC was tested by culturing with rinsed or trypsinized and replated HIV-1–exposed epithelial cells. Virus was not recovered from the rinsed or replated cocultures with resting PBMC; however, activated PBMC recovered HIV-1 from rinsed epithelial cells and rarely from replated epithelial cells. Although urogenital epithelial cells are not infected, these data suggest that they can transfer virus to activated immune cells and have implications for sexual transmission of HIV-1