In Vitro Growth Conditions Boost Plant Lipid Remodelling and Influence Their Composition

• Published in: Cells (Basel, Switzerland) Vol. 10; no. 9; p. 2326
• Main Authors: Klińska, Sylwia, Kędzierska, Sara, Jasieniecka-Gazarkiewicz, Katarzyna, Banaś, Antoni
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 06.09.2021; MDPI
• Subjects: 1-Acylglycerophosphocholine O-Acyltransferase - metabolism; Acyl Coenzyme A - metabolism; acyl-lipid metabolism; Biosynthesis; Camellia - metabolism; Comparative analysis; Enzymes; Fatty acids; Galactolipids - metabolism; Glycerol; Growth conditions; in vitro and in vivo growth conditions; Influence; Investigations; Leaves; Lipid metabolism; lipid remodelling; Lipids; Lipids - physiology; LPCAT; LPEAT; Lysophospholipids - metabolism; Membranes; Metabolites; phosphatidylcholine; Physiology; Plant Leaves - metabolism; Plastids; Seeds; Signal transduction; Substrate Specificity - physiology; LPEAT; lipid remodelling; acyl-lipid metabolism; LPCAT; in vitro and in vivo growth conditions; phosphatidylcholine
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells10092326

Acyl-lipids are vital components for all life functions of plants. They are widely studied using often conditions to determine inter alia the impact of genetic modifications and the description of biochemical and physiological functions of enzymes responsible for acyl-lipid metabolism. What is currently lacking is knowledge of if these results also hold in real environments-in conditions. Our study focused on the comparative analysis of both and growth conditions and their impact on the acyl-lipid metabolism of leaves. The results indicate that conditions significantly decreased the lipid contents and influenced their composition. In conditions, galactolipid and trienoic acid (16:3 and 18:3) contents significantly declined, indicating the impairment of the prokaryotic pathway. Discrepancies also exist in the case of acyl-CoA:lysophospholipid acyltransferases (LPLATs). Their activity increased about 2-7 times in conditions compared to . conditions also substantially changed LPLATs' preferences towards acyl-CoA. Additionally, the acyl editing process was three times more efficient in leaves. The provided evidence suggests that the results of acyl-lipid research from conditions may not completely reflect and be directly applicable in real growth environments.