Reelin Alleviates Mesenchymal Stem Cell Senescence and Reduces Pathological α-Synuclein Expression in an In Vitro Model of Parkinson's Disease

• Published in: Genes Vol. 12; no. 7; p. 1066
• Main Authors: Cho, Eunju, Park, Joonsang, Kim, Kyungri, Kim, Min-Gi, Cho, Sung-Rae
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 13.07.2021; MDPI
• Subjects: Adipose tissue; Aged; alpha-Synuclein - genetics; alpha-Synuclein - metabolism; Case-Control Studies; Cell viability; Cellular Senescence; Consent; Data analysis; Extracellular matrix; extracellular matrix-receptor related genes; Female; Gene expression; Genomes; Humans; In Vitro Techniques; Mesenchymal stem cells; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - metabolism; Microscopy; Models, Biological; Movement disorders; Neurodegeneration; Neurodegenerative diseases; Neurons; p53 Protein; Parkinson Disease - genetics; Parkinson Disease - metabolism; Parkinson Disease - pathology; Parkinson Disease - prevention & control; Parkinson's disease; Pathogenesis; Polymerase chain reaction; Reelin; Reelin protein; Reelin Protein - genetics; Reelin Protein - metabolism; Senescence; Software; Stem cells; Synuclein; Transcriptome; transcriptome analysis; Transcriptomes; United States > US; Reelin; transcriptome analysis; parkinson’s disease; extracellular matrix-receptor related genes
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes12071066

Parkinson's disease (PD) is one of the most common neurodegenerative diseases. The mechanisms underlying PD remain to be fully elucidated, and research into treatments for this condition is ongoing. Recent advances in genetic research have shed light on the mechanisms underlying PD. In this study, we used PD and control mesenchymal stem cells (MSCs) obtained from adipose tissues to confirm the differences between groups at the cellular and molecular levels. The results revealed that in PD MSCs, cell viability was clearly lower, and the rate of cell senescence was higher compared to the controls. Next, to compare the gene expression in PD and control cells, transcriptome analysis was performed. Genes in pathways, including extracellular matrix (ECM) receptor interaction, P53 signaling, and focal adhesion, were down-regulated in PD. Among genes related to ECM receptor interaction, gene expression was markedly decreased in PD cells; however, after being treated with recombinant Reelin protein, a significant increase in cell viability and a decrease in α-Synuclein aggregation and cell senescence were observed. In conclusion, Reelin affects PD by positively influencing the cell characteristics. Our findings will facilitate research into new treatments for PD.