General Patterns and Species-Specific Differences in the Organization of the Tubulin Cytoskeleton in Indeterminate Nodules of Three Legumes

• Published in: Cells (Basel, Switzerland) Vol. 10; no. 5; p. 1012
• Main Authors: Kitaeva, Anna B, Gorshkov, Artemii P, Kirichek, Evgenii A, Kusakin, Pyotr G, Tsyganova, Anna V, Tsyganov, Viktor E
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.04.2021; MDPI
• Subjects: Bacterial infections; bacteroid; Bacteroids; Cell division; Cell size; Cytoskeleton; Developmental stages; Efficiency; immunolocalization; indeterminate nodules; Infections; Legumes; legume–rhizobial symbiosis; Microtubules; Morphology; Nitrogen; Nitrogen fixation; Nodules; Scanning electron microscopy; Seeds; Species; Symbiosis; symbiosome; Transmission electron microscopy; Tubulin; United States > US; Japan; Russia; indeterminate nodules; Vicia sativa; immunolocalization; Cicer arietinum; Galega orientalis; bacteroid; microtubules; legume–rhizobial symbiosis; symbiosome
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells10051012

The tubulin cytoskeleton plays an important role in establishing legume-rhizobial symbiosis at all stages of its development. Previously, tubulin cytoskeleton organization was studied in detail in the indeterminate nodules of two legume species, and . General as well as species-specific patterns were revealed. To further the understanding of the formation of general and species-specific microtubule patterns in indeterminate nodules, the tubulin cytoskeleton organization was studied in three legume species ( , , and ). It is shown that these species differ in the shape and size of rhizobial cells (bacteroids). Immunolocalization of microtubules revealed the universality of cortical and endoplasmic microtubule organization in the meristematic cells, infected cells of the infection zone, and uninfected cells in nodules of the three species. However, there are differences in the endoplasmic microtubule organization in nitrogen-fixing cells among the species, as confirmed by quantitative analysis. It appears that the differences are linked to bacteroid morphology (both shape and size).