Polyhydroxyalkanoates-Based Nanoparticles as Essential Oil Carriers

• Published in: Polymers Vol. 14; no. 1; p. 166
• Main Authors: Corrado, Iolanda, Di Girolamo, Rocco, Regalado-González, Carlos, Pezzella, Cinzia
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.01.2022; MDPI
• Subjects: Antiinfectives and antibacterials; antimicrobial activity; Antimicrobial agents; Bacteria; Biodegradability; Biodegradation; Composition; Copolymers; Efficiency; Encapsulation; essential oil; Essential oils; Food; in vitro release; Lipids; nanoencapsulation; Nanoparticles; natural food preservatives; Oils & fats; Oregano; Particle size; Pathogens; Phenols; Polyhydroxyalkanoates; Polyhydroxybutyrate; Polymers; Mexico; nanoencapsulation; natural food preservatives; antimicrobial activity; essential oil; polyhydroxyalkanoates; in vitro release
• ISSN: 2073-4360; 2073-4360
• DOI: 10.3390/polym14010166

Plant-derived essential oils (EOs) represent a green alternative to conventional antimicrobial agents in food preservation. Due to their volatility and instability, their application is dependent on the development of efficient encapsulation strategies allowing their protection and release control. Encapsulation in Polyhydroxyalkanoate (PHA)-based nanoparticles (NPs) addresses this challenge, providing a biodegradable and biobased material whose delivery properties can be tuned by varying polymer composition. In this work, EO from Mexican oregano was efficiently encapsulated in Polyhydroxybutyrate (PHB) and Poly-3-hydroxybutyrate-co-hydroxyhexanoate (PHB-HHx)-based NPs by solvent evaporation technique achieving high encapsulation efficiency, (>60%) and loading capacity, (about 50%). The obtained NPs displayed a regular distribution with a size range of 150-210 nm. In vitro release studies in food simulant media were fitted with the Korsmeyer-Peppas model, indicating diffusion as the main factor controlling the release. The cumulative release was affected by the polymer composition, possibly related to the more amorphous nature of the copolymer, as confirmed by WAXS and DSC analyses. Both the EO-loaded nanosystems displayed antimicrobial activity against Micrococcus luteus, with PHB-HHx-based NPs being even more effective than the pure EO. The results open the way to the effective exploitation of the developed nanosystems in active packaging.