Identification of a Herpes B Virus–Specific Glycoprotein D Immunodominant Epitope Recognized by Natural and Foreign Hosts

• Published in: The Journal of infectious diseases Vol. 186; no. 4; pp. 453 - 461
• Main Authors: Perelygina, Ludmila, Zurkuhlen, Holley, Patrusheva, Irina, Hilliard, Julia K.
• Format: Journal Article
• Language: English
• Published: Chicago, IL The University of Chicago Press 15.08.2002; University of Chicago Press; Oxford University Press
• Subjects: Amino Acid Sequence; Animals; Antibodies; Antibodies, Viral - blood; Antibody Specificity; Antigens, Viral - chemistry; Antigens, Viral - genetics; Antigens, Viral - immunology; Biological and medical sciences; Blotting, Western; Cercopithecine herpesvirus 1; Enzyme-Linked Immunosorbent Assay; Epitope Mapping; Epitopes; Fundamental and applied biological sciences. Psychology; Herpesviridae Infections - diagnosis; Herpesviridae Infections - veterinary; Herpesviridae Infections - virology; Herpesvirus 1, Cercopithecine - immunology; Human herpesvirus 1; Human herpesvirus 2; Humans; Immunodominant epitopes; Immunodominant Epitopes - chemistry; Immunodominant Epitopes - genetics; Immunodominant Epitopes - immunology; Infections; Macaca; Major Articles; Microbiology; Molecular Sequence Data; Monkey Diseases - diagnosis; Monkey Diseases - virology; Neutralization Tests; Peptides - chemistry; Peptides - immunology; Proteins; Reactivity; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; Sequence Analysis, DNA; Viral Envelope Proteins - chemistry; Viral Envelope Proteins - genetics; Viral Envelope Proteins - immunology; Virology; Viruses; Infection; Virus; Antigenic determinant; Herpesviridae; Viral disease; Alphaherpesvirinae; Glycoprotein; Herpes; Immunodominance
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1086/341834

The mapping of linear epitopes of B virus (Cercopithecine herpesvirus 1 and herpes B virus) glycoprotein D (gD) was accomplished by screening the constructed gD epitope library with serum from B virus–infected macaques. The immunodominant epitope, gD (362–370), was identified within the C-terminal region of B virus gD that was highly conserved among 19 B virus clinical isolates but was not present in either herpes simplex virus (HSV)–1 or HSV-2 gD. A substantial percentage of serum samples from macaques (95%) and humans (80%) infected with B virus contained antibodies to this epitope. Antibodies against HSV types 1 or 2 did not react with this epitope; thus, gD (362–370) has unique potential to detect B virus–specific antibody responses in human serum, even in the presence of antibodies to HSV-1 and HSV-2