Experimental study on the inhibition effect of miR-106a inhibitor on tumor growth of ovarian cancer xenografts mice

• Published in: Asian Pacific journal of tropical medicine Vol. 9; no. 7; pp. 663 - 666
• Main Authors: Cai, Zhi-Hui, Chen, Li-Min, Liang, Yi-Juan, Shi, Jun-Rong, You-Ju, M.A., Wang, Wei-Ming, Yang, Huan
• Format: Journal Article
• Language: English
• Published: India Elsevier B.V 01.07.2016
• Subjects: cancer;Xenografts;miR-106a;Programmed; cell; death; miR-106a; Ovarian; Ovarian cancer; Programmed cell death 4; Xenografts; Programmed cell death 4; miR-106a; Xenografts; Ovarian cancer
• ISSN: 1995-7645; 2352-4146
• DOI: 10.1016/j.apjtm.2016.05.008

Objective:To study the inhibition effect of miR-106 a inhibitor on tumor growth of ovarian cancer xenografts mice.Methods:BALB/c mice were selected as experimental animals,ovarian cancer SKOV-3 cells transfected with miR-106 a inhibitor and its negative control were inoculated subcutaneously,intratumoral injection of miR-106 a inhibitor and its negative control were continued after tumor formation,and they were enrolled as treatment group and model group,respectively.Tumor volume and weight as well as Ki-67 and programmed cell death 4(PDCD4) expression were determined;miR-106 a inhibitor and its negative control as well as miR-106 a mimic and its negative control were transfected into SKOV-3 cells,and expression of PDCD4 in cells was determined.Results:Tumor tissue volume and weight as well as mR NA expression and protein expression of Ki-67 in treatment group were significantly lower than those in the model group while m RNA expression and protein expression of PDCD4 were significantly higher than those in the model group;transfection of mi R-106 a mimic could decrease m RNA expression and protein expression of PDCD4 in SKOV-3 cells,and transfection of miR-106 a inhibitor could increase mR NA expression and protein expression of PDCD4 in SKOV-3 cells.Conclusions:Transfection of mi R-106 a inhibitor can inhibit the growth of tumor in ovarian cancer xenografts mice through increasing the expression of PDCD4.