Adhesive Antimicrobial Peptides Containing 3,4-Dihydroxy-L-Phenylalanine Residues for Direct One-Step Surface Coating

• Published in: International journal of molecular sciences Vol. 22; no. 21; p. 11915
• Main Authors: Hwang, Young Eun, Im, Seonghun, Kim, Hyun, Sohn, Jung-Hoon, Cho, Byung-Kwan, Cho, Ju Hyun, Sung, Bong Hyun, Kim, Sun Chang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 03.11.2021; MDPI
• Subjects: Adhesion; Adhesives; Animal models; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antimicrobial agents; antimicrobial peptide; Antimicrobial peptides; Antimicrobial Peptides - chemistry; Antimicrobial Peptides - pharmacology; Bacteria; Bacterial infections; Bactericidal activity; Bond strength; C-Terminus; Coating; Coatings; Contamination; Cytotoxicity; Dihydroxyphenylalanine; E coli; Escherichia coli - drug effects; Escherichia coli - growth & development; Gram-positive bacteria; Humans; in vivo bactericidal activity; infection; Infections; L-DOPA; Peptides; Phenylalanine; Phenylalanine - chemistry; Polydimethylsiloxane; Polystyrene resins; Protective coatings; Proteins; Pseudomonas aeruginosa; Pseudomonas aeruginosa - drug effects; Pseudomonas aeruginosa - growth & development; Staphylococcus aureus - drug effects; Staphylococcus aureus - growth & development; Substrates; infection; coating; antimicrobial peptide; in vivo bactericidal activity; L-DOPA
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms222111915

Bacterial colonization and transmission via surfaces increase the risk of infection. In this study, we design and employ novel adhesive antimicrobial peptides to prevent bacterial contamination of surfaces. Repeats of 3,4-dihydroxy-L-phenylalanine (DOPA) were added to the C-terminus of NKC, a potent synthetic antimicrobial peptide, and the adhesiveness and antibacterial properties of the resulting peptides are evaluated. The peptide is successfully immobilized on polystyrene, titanium, and polydimethylsiloxane surfaces within 10 min in a one-step coating process with no prior surface functionalization. The antibacterial effectiveness of the NKC-DOPA -coated polystyrene, titanium, and polydimethylsiloxane surfaces is confirmed by complete inhibition of the growth of , , and within 2 h. The stability of the peptide coated on the substrate surface is maintained for 84 days, as confirmed by its bactericidal activity. Additionally, the NKC-DOPA -coated polystyrene, titanium, and polydimethylsiloxane surfaces show no cytotoxicity toward the human keratinocyte cell line HaCaT. The antimicrobial properties of the peptide-coated surfaces are confirmed in a subcutaneous implantation animal model. The adhesive antimicrobial peptide developed in this study exhibits potential as an antimicrobial surface-coating agent for efficiently killing a broad spectrum of bacteria on contact.