The Chemopreventive Effects of Polyphenols and Coffee, Based upon a DMBA Mouse Model with microRNA and mTOR Gene Expression Biomarkers

• Published in: Cells (Basel, Switzerland) Vol. 11; no. 8; p. 1300
• Main Authors: Molnar, Richard, Szabo, Laszlo, Tomesz, Andras, Deutsch, Arpad, Darago, Richard, Raposa, Bence L, Ghodratollah, Nowrasteh, Varjas, Timea, Nemeth, Balazs, Orsos, Zsuzsanna, Pozsgai, Eva, Szentpeteri, Jozsef L, Budan, Ferenc, Kiss, Istvan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 12.04.2022; MDPI
• Subjects: 7,12-dimethylbenz[a]anthracene; 9,10-Dimethyl-1,2-benzanthracene; 9,10-Dimethyl-1,2-benzanthracene - pharmacology; Animals; Anthracene; Anticarcinogenic Agents - pharmacology; Antioxidants; Biomarkers; Camellia sinensis; Cancer; carcinogen; Carcinogens; Chemopreventive agents; Coffea arabica; Coffee; Flavonoids; Gene Expression; Green tea; Inflammation; Interleukin 1; Interleukin 6; Kidneys; Kinases; Liver; MAP kinase; Mice; Mice, Inbred CBA; MicroRNAs - genetics; miR; miR-134; miRNA; Myc protein; Myrica rubra; NF-κB protein; Plant extracts; Polymerase chain reaction; polyphenol; Polyphenols; Polyphenols - pharmacology; Polyphenols - therapeutic use; Protein kinase; Proteins; PTEN protein; Resveratrol; Ribes nigrum; Rubus fruticosus; Spleen; Tea; TOR protein; TOR Serine-Threonine Kinases - genetics; Tumor suppressor genes; United States > US; China; miR-124-1; 7,12-dimethylbenz[a]anthracene; miR-9-3; miR-132; carcinogen; coffee; miR; miR-134; mTOR; polyphenol
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells11081300

Polyphenols are capable of decreasing cancer risk. We examined the chemopreventive effects of a green tea ( ) extract, polyphenol extract (a mixture of blackberry ( ), blackcurrants ( ), and added resveratrol phytoalexin), Chinese bayberry ( ) extract, and a coffee ( ) extract on 7,12-dimethylbenz[a]anthracene (DMBA) carcinogen-increased miR-134, miR-132, miR-124-1, miR-9-3, and gene expressions in the liver, spleen, and kidneys of CBA/Ca mice. The elevation was quenched significantly in the organs, except for miR-132 in the liver of the Chinese bayberry extract-consuming group, and miR-132 in the kidneys of the polyphenol-fed group. In the coffee extract-consuming group, only miR-9-3 and mTOR decreased significantly in the liver; also, miR-134 decreased significantly in the spleen, and, additionally, miR-124-1 decreased significantly in the kidney. Our results are supported by literature data, particularly the DMBA generated ROS-induced inflammatory and proliferative signal transducers, such as TNF, IL1, IL6, and NF-κB; as well as oncogenes, namely and . The examined chemopreventive agents, besides the obvious antioxidant and anti-inflammatory effects, mainly blocked the mentioned DMBA-activated factors and the mitogen-activated protein kinase (MAPK) as well, and, at the same time, induced as well as tumor suppressor genes.