A Review of Isothermal Amplification Methods and Food-Origin Inhibitors against Detecting Food-Borne Pathogens

The isothermal amplification method, a molecular-based diagnostic technology, such as loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA), is widely used as an alternative to the time-consuming and labor-intensive culture-based detection method. However, food...

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Published inFoods Vol. 11; no. 3; p. 322
Main Authors Moon, Ye-Ji, Lee, So-Young, Oh, Se-Wook
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 24.01.2022
MDPI
Subjects
Acids
bacteria
CRISPR
detection method
Diagnostic systems
DNA polymerase
Efficiency
Enzymes
Food
Food contamination
food metrix
Food science
Gene amplification
inhibition
Inhibitors
Magnesium
molecular amplification
Pathogens
Polyethylene glycol
Polyphenols
Polysaccharides
Proteins
Recombinase
Review
RNA polymerase
Saccharides
Seafood
food metrix
inhibition
bacteria
detection method
molecular amplification
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Summary:The isothermal amplification method, a molecular-based diagnostic technology, such as loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA), is widely used as an alternative to the time-consuming and labor-intensive culture-based detection method. However, food matrices or other compounds can inhibit molecular-based diagnostic technologies, causing reduced detection efficiencies, and false-negative results. These inhibitors originating from food are polysaccharides and polyphenolic compounds in berries, seafood, and vegetables. Additionally, magnesium ions needed for amplification reactions can also inhibit molecular-based diagnostics. The successful removal of inhibitors originating from food and molecular amplification reaction is therefore proposed to enhance the efficiency of molecular-based diagnostics and allow accurate detection of food-borne pathogens. Among molecular-based diagnostics, PCR inhibitors have been reported. Nevertheless, reports on the mechanism and removal of isothermal amplification method inhibitors are insufficient. Therefore, this review describes inhibitors originating from food and some compounds inhibiting the detection of food-borne pathogens during isothermal amplification.
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ISSN:2304-8158
2304-8158
DOI:10.3390/foods11030322