The time-course of appearance and net accumulation of horseradish peroxidase (HRP) presented orally to rainbow trout Salmo gairdneri (Richardson)

• Published in: Comparative biochemistry and physiology. A, Comparative physiology Vol. 88; no. 3; pp. 507 - 510
• Main Authors: Mclean, E, Ash, R
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier B.V 1987; Elsevier
• Subjects: Animals; Applied sciences; Biological and medical sciences; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Horseradish Peroxidase - pharmacokinetics; Intestinal Absorption; Intestine. Mesentery; Other techniques and industries; Peroxidases - pharmacokinetics; Salmonidae - metabolism; Time Factors; Tissue Distribution; Trout - metabolism; Vertebrates: digestive system; Freshwater environment; Proteins; Vertebrata; Enzyme; Pisces; Gut; Digestion; Salmo gairdneri; Biological accumulation; ELISA assay
• ISSN: 0300-9629
• DOI: 10.1016/0300-9629(87)90072-7

1. 1. A sensitive enzyme-linked immunosorbent assay (ELISA) technique was used in order to determine horseradish peroxidase (HRP) uptake from the rainbow trout gut. 2. 2. HRP was detected in blood plasma and various tissues within 15 min of oral intubation. 3. 3. The time-course of net accumulation (uptake-degradatation) over a 75 min period was recorded. 4. 4. The presence of HRP reached a maximum in the body tissues approximately 45 min after intubation and on a ng/g wet weight basis the order of accumulation within the tissues was liver > spleen = kidney > plasma > heart. 5. 5. The total organ accumulation (net) was in the order liver > plasma > kidney > spleen > heart.