RgC3H Involves in the Biosynthesis of Allelopathic Phenolic Acids and Alters Their Release Amount in Rehmannia glutinosa Roots

• Published in: Plants (Basel) Vol. 9; no. 5; p. 567
• Main Authors: Yang, Yanhui, Zhang, Zhongyi, Li, Ruifang, Yi, Yanjie, Yang, Heng, Wang, Chaojie, Wang, Zushiqi, Liu, Yunyi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 29.04.2020; MDPI
• Subjects: allelopathic compounds; Allelopathy; Amino acids; Biosynthesis; Exudates; Exudation; Herbal medicine; Hydroxylase; Localization; p-Coumarate-3-hydroxylase; Phenolic acid biosynthesis; Phenolic acids; Phenols; Proteins; R. glutinosa; Raw materials; Rehmannia glutinosa; release amount; root exudates; Roots; p-Coumarate-3-hydroxylase; Phenolic acid biosynthesis; release amount; R. glutinosa; root exudates; allelopathic compounds
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants9050567

production is affected by replanting disease, in which autotoxic harm to plants is mediated by endogenous phenolic acids as allelopathic compounds found in root exudates. These phenolic acids are mostly phenylpropanoid products of plants' secondary metabolisms. The molecular mechanism of their biosynthesis and release has not been explored in . -coumarate-3-hydroxylase (C3H) is the second hydroxylase gene involved in the phenolic acid/phenylpropanoid biosynthesis pathways. C3Hs have been functionally characterized in several plants. However, limited information is available on the gene in . Here, we identified a putative gene and predicted its potential function by in silico analysis and subcellular localization. Overexpression or repression of in the transgenic roots indicated that the gene was involved in allelopathic phenolic biosynthesis. Moreover, we found that these phenolic acid release amount of the transgenic roots were altered, implying that positively promotes their release via the molecular networks of the activated phenolic acid/phenylpropanoid pathways. This study revealed that plays roles in the biosynthesis and release of allelopathic phenolic acids in roots, laying a basis for further clarifying the molecular mechanism of the replanting disease development.