Detection of ricin intoxication in mice using serum peptide profiling by MALDI-TOF/MS

• Published in: International journal of molecular sciences Vol. 13; no. 10; pp. 13704 - 13712
• Main Authors: Zhao, Siyan, Liu, Wen-Sen, Wang, Meng, Li, Jiping, Sun, Yucheng, Li, Nan, Hou, Feng, Wan, Jia-Yu, Li, Zhongyi, Qian, Jun, Liu, Linna
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22.10.2012; Molecular Diversity Preservation International (MDPI)
• Subjects: Accuracy; Animals; Biomarkers; Biomarkers - blood; detection model; Female; Infections; Injections, Intraperitoneal; Intoxication; Magnetics; MALDI-TOF-MS; Mass spectrometry; Mice; Mice, Inbred BALB C; Peptides; Peptides - blood; Protein synthesis; Proteins; Ribonucleic acid; ricin; Ricin - metabolism; RNA; Scientific imaging; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Time Factors; Vaccines; China
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms131013704

Ricin toxin has been regarded as one of the most potent poisons in the plant kingdom, and there is no effective therapeutic countermeasure or licensed vaccine against it. Consequently, early detection of ricin intoxication is necessary. In this study, we took mice as test subjects, and used the technique of Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/MS) and ClinProt™ microparticle beads to set up an effective detection model with an accuracy of almost 100%. Eighty-two peaks in the mass range 1000-10,000 m/z were detected by ClinProTools software, and five different peaks with m/z of 4982.49, 1333.25, 1537.86, 4285.05 and 2738.88 had the greatest contribution to the accuracy and sensitivity of this model. They may therefore provide biomarkers for ricin intoxication.