Histone H3Q5 serotonylation stabilizes H3K4 methylation and potentiates its readout

Serotonylation of glutamine 5 on histone H3 (H3Q5ser) was recently identified as a permissive posttranslational modification that coexists with adjacent lysine 4 trimethylation (H3K4me3). While the resulting dual modification, H3K4me3Q5ser, is enriched at regions of active gene expression in seroton...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 118; no. 6; pp. 1 - 7
Main Authors Zhao, Shuai, Chuh, Kelly N., Zhang, Baichao, Dul, Barbara E., Thompson, Robert E., Farrelly, Lorna A., Liu, Xiaohui, Xu, Ning, Xue, Yi, Roeder, Robert G., Maze, Ian, Muir, Tom W., Li, Haitao
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 09.02.2021
Subjects
Biological Sciences
Chromatin - genetics
Glutamine - genetics
Glutamine - metabolism
Histones - genetics
Histones - metabolism
Humans
Lysine - genetics
Methylation
Protein Binding - genetics
Protein Processing, Post-Translational - genetics
Serotonergic Neurons - metabolism
designer chromatin
histone modification
modification crosstalk
H3Q5 serotonylation
H3K4me3
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Summary:Serotonylation of glutamine 5 on histone H3 (H3Q5ser) was recently identified as a permissive posttranslational modification that coexists with adjacent lysine 4 trimethylation (H3K4me3). While the resulting dual modification, H3K4me3Q5ser, is enriched at regions of active gene expression in serotonergic neurons, the molecular outcome underlying H3K4me3–H3Q5ser crosstalk remains largely unexplored. Herein, we examine the impact of H3Q5ser on the readers, writers, and erasers of H3K4me3. All tested H3K4me3 readers retain binding to the H3K4me3Q5ser dual modification. Of note, the PHD finger of TAF3 favors H3K4me3Q5ser, and this binding preference is dependent on the Q5ser modification regardless of H3K4 methylation states. While the activity of the H3K4 methyltransferase, MLL1, is unaffected by H3Q5ser, the corresponding H3K4me3/2 erasers, KDM5B/C and LSD1, are profoundly inhibited by the presence of the mark. Collectively, this work suggests that adjacent H3Q5ser potentiates H3K4me3 function by either stabilizing H3K4me3 from dynamic turnover or enhancing its physical readout by downstream effectors, thereby potentially providing a mechanism for fine-tuning critical gene expression programs.
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1S.Z., K.N.C., and B.Z. contributed equally to this work.
Author contributions: I.M., T.W.M., and H.L. conceived research; S.Z., K.N.C., and B.Z. designed research; S.Z., K.N.C., and B.Z. performed research; B.E.D., R.E.T., L.A.F., X.L., N.X., Y.X., and R.G.R. contributed new reagents/analytic tools; S.Z., K.N.C., B.Z., T.W.M., and H.L. analyzed data; and S.Z., K.N.C., B.Z., I.M., T.W.M., and H.L. wrote the paper.
Edited by Peter Cheung, York University, Toronto, ON, Canada, and accepted by Editorial Board Member Karolin Luger November 12, 2020 (received for review August 7, 2020)
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.2016742118