The role of lysyl oxidase-like 2 in the odontogenic differentiation of human dental pulp stem cells

• Published in: Molecules and cells Vol. 35; no. 6; pp. 543 - 549
• Main Authors: Kim, J.H., Seoul National University, Seoul, Republic of Korea, Lee, E.H., Kyungpook National University, Daegu, Republic of Korea, Park, H.J., Seoul National University, Seoul, Republic of Korea, Park, E.K., Kyungpook National University, Daegu, Republic of Korea, Kown, T.G., Kyungpook National University, Daegu, Republic of Korea, Shin, H.I., Kyungpook National University, Daegu, Republic of Korea, Cho, J.Y., Seoul National University, Seoul, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.06.2013; Korean Society for Molecular and Cellular Biology; Korea Society for Molecular and Cellular Biology; 한국분자세포생물학회
• Subjects: Adult Stem Cells - drug effects; Adult Stem Cells - physiology; Amino Acid Oxidoreductases - genetics; Amino Acid Oxidoreductases - metabolism; Amino Acid Oxidoreductases - pharmacology; Biochemistry; Biomarkers - metabolism; Biomedical and Life Sciences; Biomedicine; Biotechnology; Calcification, Physiologic - drug effects; Cell Biology; Cell Differentiation - drug effects; Cell Differentiation - genetics; Cells, Cultured; Chemokine CCL27 - metabolism; Dental Pulp - drug effects; Dental Pulp - physiology; Extracellular Matrix Proteins - metabolism; Gene Expression Regulation, Developmental - drug effects; Gene Expression Regulation, Developmental - genetics; human dental pulp stem cells, lysyl oxidase-like 2, odontogenic differentiation; Humans; Life Sciences; Odontogenesis - drug effects; Odontogenesis - genetics; OXIDOREDUCTASES; OXIDORREDUCTASAS; OXYDOREDUCTASE; Phosphoproteins - metabolism; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Recombinant Proteins - pharmacology; Research Article; Sialoglycoproteins - metabolism; Stem Cell Niche; 생물학; odontogenic differentiation; lysyl oxidase-like 2; human dental pulp stem cells
• ISSN: 1016-8478; 0219-1032
• DOI: 10.1007/s10059-013-0080-3

Adult human dental pulp stem cells (hDPSCs) are a unique population of precursor cells those are isolated from postnatal dental pulp and have the ability to differentiate into a variety of cell types utilized for the formation of a reparative dentin-like complex. Using LC-MS/MS proteomics approaches, we identified the proteins secreted from the differentiating hDPSCs in mineralization media. Lysyl oxidase- like 2 (LOXL2) was identified as a protein that was down-regulated in the hDPSCs that differentiate into odontoblast- like cells. The role of LOXL2 has not been studied in dental pulp stem cells. LOXL2 mRNA levels were reduced in differentiating hDPSCs, whereas the levels of other LOX family members including LOX, LOXL1, LOXL3, and LOXL4, are increased. The protein expression and secretion levels of LOXL2 were also decreased during odontogenic differentiation. Recombinant LOXL2 protein treatment to hDPSCs resulted in a dose-dependent decrease in the early differentiation and the mineralization accompanying with the lower levels of odontogenic markers such as DSPP, DMP-1 and ALP. These results suggest that LOXL2 has a negative effect on the differentiation of hDPSCs and blocking LOXL2 can promote the hDPSC differentiation to odontoblasts.