Altered Phenotype and Enhanced Antibody-Producing Ability of Peripheral B Cells in Mice with Cd19 -Driven Cre Expression
Given the importance of B lymphocytes in inflammation and immune defense against pathogens, mice transgenic for Cre under the control of promoter ( mice) have been widely used to specifically investigate the role of -flanked genes in B cell development/function. However, impacts of expression/insert...
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Published in | Cells (Basel, Switzerland) Vol. 11; no. 4; p. 700 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI AG
16.02.2022
MDPI |
Subjects | |
Online Access | Get full text |
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Summary: | Given the importance of B lymphocytes in inflammation and immune defense against pathogens, mice transgenic for Cre under the control of
promoter (
mice) have been widely used to specifically investigate the role of
-flanked genes in B cell development/function. However, impacts of expression/insertion of the Cre transgene on the phenotype and function of B cells have not been carefully studied. Here, we show that the number of marginal zone B and B1a cells was selectively reduced in
mice, while B cell development in the bone marrow and total numbers of peripheral B cells were comparable between
and wild type C57BL/6 mice. Notably, humoral responses to both T cell-dependent and independent antigens were significantly increased in
mice. We speculate that these differences are mainly attributable to reduced surface CD19 levels caused by integration of the Cre-expressing cassette that inactivates one
allele. Moreover, our literature survey showed that expression of
alone may affect the development/progression of inflammatory and anti-infectious responses. Thus, our results have important implications for the design and interpretation of results on gene functions specifically targeted in B cells in the
mouse strain, for instance, in the context of (auto) inflammatory/infectious diseases. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2073-4409 2073-4409 |
DOI: | 10.3390/cells11040700 |