SPR-based immunosensor for the CRP detection—A new method to detect a well known protein

• Published in: Biosensors & bioelectronics Vol. 21; no. 10; pp. 1987 - 1990
• Main Authors: Meyer, Martin H.F., Hartmann, Markus, Keusgen, Michael
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 15.04.2006; Elsevier Science
• Subjects: Antibodies; AntiCRP clone C2 and C6; Biological and medical sciences; Biosensing Techniques; Biosensors; Biotechnology; C-Reactive Protein - analysis; C-Reactive Protein - immunology; C-Reactive Protein - metabolism; CRP; Enzyme-Linked Immunosorbent Assay; Fundamental and applied biological sciences. Psychology; Humans; Immunosensor; Inflammation; Methods. Procedures. Technologies; SPR; Surface Plasmon Resonance; Various methods and equipments; CRP; Inflammation; SPR; Immunosensor; AntiCRP clone C2 and C6; Biosensor; Resonance; Method; Detection; Surface plasmon; C reactive protein
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2005.09.010

The c-reactive protein (CRP) is one of the significant human blood serum markers for inflammatory processes. The serum presence of this hepatic ∼115 kDa protein of five identical subunits accompanies several diseases (e.g. CVD, inflammatory bowel diseases) and is nowadays detected by high-sensitivity ELISA assays in blood serum. To enable CRP detection in other matrices, an SPR-based (surface plasmon resonance) immunosensor for the CRP detection has been established. A linear detection range of 2–5 μg CRP per ml was found, using two different antiCRP antibodies (monoclonal, IgG) for CRP trapment and detection. Furthermore, the kinetic antibody association and dissociation constants of one antibody (antiCRP, clone C2) could be determined.