High-performance liquid chromatographic method for the determination of dolichols in tissues and plasma

• Published in: Analytical biochemistry Vol. 150; no. 1; pp. 26 - 31
• Main Authors: Yamada, Kouji, Yokohama, Hiromitsu, Abe, Shinya, Katayama, Kouichi, Sato, Tadashi
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.10.1985; Elsevier
• Subjects: Adult; Analytical, structural and metabolic biochemistry; Animals; Applied sciences; Biological and medical sciences; Chromatography, High Pressure Liquid; Diterpenes - analysis; dolichol; Dolichol - analogs & derivatives; Dolichol - analysis; Dolichol - blood; Exact sciences and technology; fluorometric method; Fundamental and applied biological sciences. Psychology; high-performance liquid chromatography; Humans; Intermediary metabolites. Miscellaneous; Male; Mass Spectrometry; Middle Aged; Other biological molecules; Other techniques and industries; plasma level; Rats; Rats, Inbred Strains; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; tissue level; dolichol; plasma level; high-performance liquid chromatography; tissue level; fluorometric method; Assay; Tissue; HPLC chromatography; Detection; Ultraviolet spectrometry; Fluorescence spectrometry; Blood plasma
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/0003-2697(85)90437-3

High-performance liquid chromatographic methods for the determination of dolichols in tissues and plasma have been developed. The tissue concentration of dolichols was measured by high-performance liquid chromatography with uv detection and plasma levels of dolichols were determined fluorometrically after derivatization with anthracene-9-carboxylic acid. In both methods, 2,2-didecaprenylethanol was used as an internal standard. The method with fluorescence detection was sufficiently sensitive to measure the concentration of dolichols in human plasma.