Induction of ornithine decarboxylase in guinea-pig lymphocytes and its relation to phospholipid metabolism

• Published in: Biochimica et Biophysica Acta (BBA) - General Subjects Vol. 800; no. 1; pp. 96 - 101
• Main Authors: Otani, Shuzo, Matsui, Isao, Kuramoto, Ayako, Morisawa, Seiji
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 16.07.1984; Elsevier BV; Elsevier; North-Holland
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Bucladesine; Bucladesine - pharmacology; Calcimycin; Calcimycin - pharmacology; Clostridium perfringens; Clostridium perfringens - enzymology; Diglycerides; Diglycerides - biosynthesis; Diphosphates; Diphosphates - metabolism; Enzyme Induction; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Guinea Pigs; Guinea-pig lymphocyte; Kinetics; Lyases; Lymph Nodes; Lymph Nodes - metabolism; Lymphocyte Activation; Lymphocytes; Lymphocytes - drug effects; Lymphocytes - immunology; Lymphocytes - metabolism; Ornithine Decarboxylase; Ornithine Decarboxylase - biosynthesis; Phospholipid metabolism; Phospholipids; Phospholipids - biosynthesis; Type C Phospholipases; Type C Phospholipases - metabolism; Enzyme induction; Hepes; Guinea-pig lymphocyte; Ornithine decarboxylase; Phospholipid metabolism; POPOP; PPO; Enzyme; Rodentia; Clostridiales; Phospholipid; Activation; Metabolism; Clostridium perfringens; Phospholipase C; Vertebrata; Mammalia; Guinea pig; Clostridiaceae; Bacteria; Lymphocyte
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/0304-4165(84)90098-9

Treatment of lymphocytes with exogenous phospholipase C (phosphatidylcholine cholinephosphohydrolase, EC 3.1.4.3.) derived from Clostridium perfringens at concentrations similar to those which induced ornithine decarboxylase ( L-ornithine carboxy-lyase, EC 4.1.1.17) activity produced diacylglycerol and phosphatidate. A divalent cation ionophore, A23187, and phytohemagglutinin induced not only diacylglycerol formation, but also ornithine decarboxylase activity. Dibutyryl cAMP inhibited both diacylglycerol formation and ornithine decarboxylase induction to a similar extent in phytohemagglutinin-stimulated lymphocytes, but stimulated them somewhat in ionophore A23187-activated lymphocytes. This suggests that the activation of intracellular phospholipase C and the formation of diacylglycerol is involved in ornithine decarboxylase induction in lymphocytes.