Single Cell Effects of Photobiomodulation on Mitochondrial Membrane Potential and Reactive Oxygen Species Production in Human Adipose Mesenchymal Stem Cells

• Published in: Cells (Basel, Switzerland) Vol. 11; no. 6; p. 972
• Main Authors: Pan, Li-Chern, Hang, Nguyen-Le-Thanh, Colley, Mamadi M S, Chang, Jungshan, Hsiao, Yu-Cheng, Lu, Long-Sheng, Li, Bing-Sian, Chang, Cheng-Jen, Yang, Tzu-Sen
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11.03.2022; MDPI
• Subjects: Adenosine triphosphate; Adipose Tissue - metabolism; Cell culture; Cell differentiation; Cell growth; Cell migration; Cell proliferation; Cell Survival; Cytochrome; human adipose-derived mesenchymal stem cell; Humans; I.R. radiation; Investigations; Lasers; Membrane potential; Membrane Potential, Mitochondrial; Mesenchymal stem cells; Mesenchymal Stem Cells - metabolism; Microscopy; Mitochondria; mitochondrial membrane potential; photobiomodulation; Reactive oxygen species; Reactive Oxygen Species - metabolism; Rhodamine; Stem cells; vesicle transport; United States > US; Japan; Germany; photobiomodulation; vesicle transport; human adipose-derived mesenchymal stem cell; mitochondrial membrane potential; reactive oxygen species
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells11060972

Photobiomodulation (PBM) has recently emerged in cellular therapy as a potent alternative in promoting cell proliferation, migration, and differentiation during tissue regeneration. Herein, a single-cell near-infrared (NIR) laser irradiation system (830 nm) and the image-based approaches were proposed for the investigation of the modulatory effects in mitochondrial membrane potential (ΔΨm), reactive oxygen species (ROS), and vesicle transport in single living human adipose mesenchymal stem cells (hADSCs). The irradiated-hADSCs were then stained with 2',7'-dichlorodihydrofluorescein diacetate (H DCFDA) and Rhodamine 123 (Rh123) to represent the ΔΨm and ROS production, respectively, with irradiation in the range of 2.5-10 (J/cm ), where time series of bright-field images were obtained to determine the vesicle transport phenomena. Present results showed that a fluence of 5 J/cm of PBM significantly enhanced the ΔΨm, ROS, and vesicle transport phenomena compared to the control group (0 J/cm ) after 30 min PBM treatment. These findings demonstrate the efficacy and use of PBM in regulating ΔΨm, ROS, and vesicle transport, which have potential in cell proliferation, migration, and differentiation in cell-based therapy.