Study on the anti-inflammatory activity of the porcine bone collagen peptides prepared by ultrasound-assisted enzymatic hydrolysis

• Published in: Ultrasonics sonochemistry Vol. 101; p. 106697
• Main Authors: Hao, Yuejing, Xing, Lujuan, Wang, Zixu, Cai, Jiaming, Toldrá, Fidel, Zhang, Wangang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier 01.12.2023
• Subjects: Animals; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Anti-inflammatory effect; Antioxidants - chemistry; Bioactive peptides; Chromatography, Liquid; Collagen - chemistry; Collagen - pharmacology; Enzymatic hydrolysis; Hydrolysis; Molecular Docking Simulation; Original; Peptides - chemistry; Peptides - pharmacology; Porcine bone collagen; Swine; Tandem Mass Spectrometry; Ultrasound; Anti-inflammatory effect; Porcine bone collagen; Bioactive peptides; Ultrasound; Enzymatic hydrolysis
• ISSN: 1350-4177; 1873-2828
• DOI: 10.1016/j.ultsonch.2023.106697

In this study, the effects of ultrasound-assisted enzymatic hydrolysis on the extraction of anti-inflammatory peptides from porcine bone collagen were investigated. The results showed that ultrasound treatment increased the content of α-helix while decreased β-chain and random coil, promoted generation of small molecular peptides. Ultrasound-assisted enzymatic hydrolysis improved the peptide content, enhanced ABTS radical scavenging and ferrous ion chelating ability than non-ultrasound group. At the ultrasonic power of 450 W (20 min), peptides possessed significant anti-inflammatory activity, where the releasing of interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) was all suppressed in lipopolysaccharide (LPS) induced RAW264.7 cells. After the analysis with LC-MS/MS, eight peptides with potential anti-inflammatory activities were selected by the PeptideRanker and molecular docking. In general, the ultrasound-assisted enzymatic hydrolysis was an effective strategy to extract the bioactive peptides from porcine bone, and the inflammatory regulation capacity of bone collagen sourced peptides was firstly demonstrated.