Defining the Skeletal Myogenic Lineage in Human Pluripotent Stem Cell-Derived Teratomas

Skeletal muscle stem cells are essential to muscle homeostasis and regeneration after injury, and have emerged as a promising cell source for treating skeletal disorders. An attractive approach to obtain these cells utilizes differentiation of pluripotent stem cells (PSCs). We recently reported that...

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Published inCells (Basel, Switzerland) Vol. 11; no. 9; p. 1589
Main Authors Pappas, Matthew P, Xie, Ning, Penaloza, Jacqueline S, Chan, Sunny S K
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 09.05.2022
MDPI
Subjects
Animals
Cell culture
Cell differentiation
Correlation analysis
Developmental stages
Embryos
ErbB-3 protein
Gene expression
Genomics
Homeostasis
Humans
Mice
Muscle Development - physiology
Muscle Fibers, Skeletal - metabolism
muscle stem cells
Muscle, Skeletal
Musculoskeletal system
Myogenesis
myogenic development
Pluripotency
pluripotent stem cells
Pluripotent Stem Cells - metabolism
Population
Principal components analysis
Progenitor cells
satellite cells
Skeletal muscle
Stem cell transplantation
Stem cells
Surface markers
Teratoma
Transcriptomics
Minneapolis Minnesota
United States > US
muscle stem cells
pluripotent stem cells
myogenic development
satellite cells
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Summary:Skeletal muscle stem cells are essential to muscle homeostasis and regeneration after injury, and have emerged as a promising cell source for treating skeletal disorders. An attractive approach to obtain these cells utilizes differentiation of pluripotent stem cells (PSCs). We recently reported that teratomas derived from mouse PSCs are a rich source of skeletal muscle stem cells. Here, we showed that teratoma formation is also capable of producing skeletal myogenic progenitors from human PSCs. Using single-cell transcriptomics, we discovered several distinct skeletal myogenic subpopulations that represent progressive developmental stages of the skeletal myogenic lineage and recapitulate human embryonic skeletal myogenesis. We further discovered that ERBB3 and CD82 are effective surface markers for prospective isolation of the skeletal myogenic lineage in human PSC-derived teratomas. Therefore, teratoma formation provides an accessible model for obtaining human skeletal myogenic progenitors from PSCs.
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These authors contributed equally to this work.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells11091589