Antioxidant properties of Baccharis trimera in the neutrophils of Fisher rats

• Published in: Journal of ethnopharmacology Vol. 129; no. 3; pp. 381 - 386
• Main Authors: Pádua, Bruno da Cruz, Silva, Lucas Dornela, Rossoni, Joamyr Victor, Humberto, Jorge Luiz, Chaves, Míriam Martins, Silva, Marcelo Eustáquio, Pedrosa, Maria Lucia, Costa, Daniela Caldeira
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 16.06.2010; Amsterdam; New York: Elsevier; Elsevier
• Subjects: acetaminophen; Acetaminophen - pharmacology; Alanine Transaminase - blood; animal models; Animals; Antioxidant; antioxidant activity; antioxidants; Antioxidants - isolation & purification; Antioxidants - pharmacology; Aspartate Aminotransferases - blood; Asteraceae; Baccharis; Baccharis - chemistry; Baccharis trimera; Biological and medical sciences; Cells, Cultured; chemical concentration; cultured cells; dosage; dose response; Dose-Response Relationship, Drug; General pharmacology; Male; Medical sciences; medicinal plants; medicinal properties; Neutrophils; Neutrophils - drug effects; Neutrophils - enzymology; Neutrophils - metabolism; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plant Components, Aerial - chemistry; plant extracts; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Quercetin - pharmacology; Rats; Rats, Inbred F344; Reactive oxygen species; Reactive Oxygen Species - metabolism; Time Factors; Zymosan - pharmacology; Brazil; South America; America; Reactive oxygen species; Baccharis trimera; Antioxidant; Neutrophils; Rat; Pharmacognosy; Rodentia; Compositae; Medicinal plant; Vertebrata; Mammalia; Folk medicine; Dicotyledones; Animal; Angiospermae; Plant origin; Spermatophyta; Neutrophil
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2010.04.018

In vitro, the hydroethanolic extract of Baccharis trimera 5.0 μg/mL (Bt5) reduced the release of reactive oxygen species (RLU/30 min) in neutrophils, even after stimulation with zymosan (ZC3b) (A). In vivo, Baccharis trimera crude extract 600 mg/kg body weight (Bt) significantly reduced ROS production in the neutrophils of animals intoxicated with acetaminophen (APAP). This same effect was observed for quercetin 50 mg/kg body weight (Que) – reference substance (B). Baccharis trimera (Less.) (Asteraceae) is a native plant of Brazil. Also known as “carqueja”, it has been popularly used to treat liver diseases, diabetes, as well as digestive disorders. Other studies have described the hepatoprotective, antioxidant and anti-inflammatory activities of the species. The aim of the present study was to investigate the antioxidant properties of Baccharis trimera in the neutrophils of Fisher rats in both in vitro and in vivo experimental models. In the in vitro assay, the neutrophils of male rats were isolated and incubated with Baccharis trimera extract at concentrations of 0.5, 5.0 and 50.0 μg/mL. In the in vivo assay, male rats were first treated with crude extract 600 mg/kg body weight of Baccharis trimera or with 50 mg/kg body weight of quercetin (reference substance) and then treated with 835 mg/kg of acetaminophen (APAP) after 24 h. The hydroethanolic extract of Baccharis trimera reduced the release of reactive oxygen species in the neutrophils in both the in vitro and in vivo experimental models. Therefore confirming its antioxidant effect. The results of this study confirm the antioxidant effect of Baccharis trimera.