Isolation of mesotrione-degrading bacteria from aquatic environments in Brazil

• Published in: Chemosphere (Oxford) Vol. 86; no. 11; pp. 1127 - 1132
• Main Authors: Pileggi, Marcos, Pileggi, Sônia Alvim Veiga, Olchanheski, Luiz Ricardo, da Silva, Paulo Augusto Garbugio, Munoz Gonzalez, Ana M., Koskinen, Willian C., Barber, Brian, Sadowsky, Michael Jay
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.03.2012; Elsevier
• Subjects: Applied sciences; aquatic environment; bacteria; Bacteria - genetics; Bacteria - isolation & purification; Bacteria - metabolism; benzoic acid; Biodegradation; Biodegradation, Environmental; Biological and medical sciences; Biological and physicochemical phenomena; Biological treatment of waters; Bioremediation; Biotechnology; Brazil; Callisto; corn; Cyclohexanones - analysis; Cyclohexanones - metabolism; Earth sciences; Earth, ocean, space; Engineering and environment geology. Geothermics; Environment and pollution; Exact sciences and technology; Fresh Water - chemistry; Fresh Water - microbiology; Fundamental and applied biological sciences. Psychology; glucose; Herbicides - analysis; Herbicides - metabolism; Industrial applications and implications. Economical aspects; mesotrione; metabolism; Natural water pollution; Pantoea - genetics; Pantoea - isolation & purification; Pantoea - metabolism; Pantoea ananatis; Pollution; Pollution, environment geology; Water Pollutants, Chemical - analysis; Water Pollutants, Chemical - metabolism; Water treatment and pollution; Xenobiotic; Brazil; South America; America; Biodegradation; Callisto; Pantoea ananatis; Bioremediation; Xenobiotic; Microbial activity; Agricultural chemical product; Pesticides; Liquid chromatography; Herbicide; Chemical pollution; Decontamination; Mass spectrometry MS/MS; Bacteria; Water pollution; Organic compounds
• ISSN: 0045-6535; 1879-1298; 1879-1298
• DOI: 10.1016/j.chemosphere.2011.12.041

► Pantoea ananatis was isolated in a Brazilian river near mesotrione treated fields. ► This bacterium completely degrades mesotrione without producing toxic substances. ► P. ananatis does not use mesotrione as carbon, nitrogen or sulfur source. ► The degradation probably goes through a cometabolic pathway. ► Callisto® adjutants prevent P. ananatis growth in herbicide high concentrations. Mesotrione is a benzoylcyclohexane-1,3-dione herbicide that inhibits 4-hydroxyphenyl pyruvate dioxygenase in target plants. Although it has been used since 2000, only a limited number of degrading microorganisms have been reported. Mesotrione-degrading bacteria were selected among strains isolated from Brazilian aquatic environments, located near corn fields treated with this herbicide. Pantoea ananatis was found to rapidly and completely degrade mesotrione. Mesotrione did not serve as a sole C, N, or S source for growth of P. ananatis, and mesotrione catabolism required glucose supplementation to minimal media. LC–MS/MS analyses indicated that mesotrione degradation produced intermediates other than 2-amino-4-methylsulfonyl benzoic acid or 4-methylsulfonyl-2-nitrobenzoic acid, two metabolites previously identified in a mesotrione-degrading Bacillus strain. Since P. ananatis rapidly degraded mesotrione, this strain might be useful for bioremediation purposes.