Advances in directed molecular evolution of reporter genes

Many reporter genes, such as gfp, gusA, and lacZ, are widely used for research into plants, animals, and microorganisms. Reporter genes, which offer high levels of sensitivity and convenience of detection, have been utilized in transgenic technology, promoter analysis, drug screening, and other area...

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Published inCritical reviews in biotechnology Vol. 32; no. 2; pp. 133 - 142
Main Authors Xiong, Ai-Sheng, Peng, Ri-He, Zhuang, Jing, Davies, Jim, Zhang, Jian, Yao, Quan-Hong
Format Journal Article
LanguageEnglish
Published London Informa Healthcare 01.06.2012
Taylor & Francis
Subjects
Animals
beta-Galactosidase - genetics
biocatalysts
Biological and medical sciences
Biotechnology
Directed evolution
Directed Molecular Evolution
DNA shuffling
drugs
fluorescent protein
Fundamental and applied biological sciences. Psychology
Genes, Reporter
Glucuronidase - genetics
Green Fluorescent Proteins - genetics
Humans
Methods. Procedures. Technologies
microorganisms
plants (botany)
polymerase chain reaction
Protein engineering
protein structure
proteins
reporter gene
reporter genes
screening
β-galactosidase
β-glucuronidase
Glycosylases
Directed molecular evolution
fluorescent protein
β-Glucuronidase
Reporter gene
Directed evolution
Enzyme
Glycosidases
Fluorescence
Hydrolases
Protein
β-Galactosidase
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Summary:Many reporter genes, such as gfp, gusA, and lacZ, are widely used for research into plants, animals, and microorganisms. Reporter genes, which offer high levels of sensitivity and convenience of detection, have been utilized in transgenic technology, promoter analysis, drug screening, and other areas. Directed molecular evolution is a powerful molecular tool for the creation of designer proteins for industrial and research applications, including studies of protein structure and function. Directed molecular evolution is based mainly on in vitro recombination methods, such as error-prone PCR and DNA shuffling. The strategies of directed evolution of enzyme biocatalysts have been the subject of several recent reviews. Here, we briefly summarize successes in the field of directed molecular evolution of reporter genes and discuss some of the applications.
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ISSN:0738-8551
1549-7801
1549-7801
DOI:10.3109/07388551.2011.593503