Linkage between N2O emission and functional gene abundance in an intensively managed calcareous fluvo-aquic soil

The linkage between N 2 O emissions and the abundance of nitrifier and denitrifier genes is unclear in the intensively managed calcareous fluvo-aquic soils of the North China Plain. We investigated the abundance of bacterial amoA for nitrification and narG, nirS, nirK , and nosZ for denitrification...

Full description

Saved in:
Bibliographic Details
Published inScientific reports Vol. 7; no. 1; p. 43283
Main Authors Yang, Liuqing, Zhang, Xiaojun, Ju, Xiaotang
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 24.02.2017
Nature Publishing Group
Subjects
704/106/694
704/47/4112
Abundance
Agricultural practices
AmoA gene
Calcareous soils
Crop rotation
Denitrification
Emission measurements
Emissions
Fertilization
Fertilizer application
Genes
Humanities and Social Sciences
Manures
multidisciplinary
NirK protein
Nitrification
Nitrous oxide
Science
Straw
Urea
Online AccessGet full text

Cover

More Information
Summary:The linkage between N 2 O emissions and the abundance of nitrifier and denitrifier genes is unclear in the intensively managed calcareous fluvo-aquic soils of the North China Plain. We investigated the abundance of bacterial amoA for nitrification and narG, nirS, nirK , and nosZ for denitrification by in situ soil sampling to determine how the abundance of these genes changes instantly during N fertilization events and is related to high N 2 O emission peaks. We also investigated how long-term incorporated straw and/or manure affect(s) the abundance of these genes based on a seven-year field experiment. The overall results demonstrate that the long-term application of urea-based fertilizer and/or manure significantly enhanced the number of bacterial amoA gene copies leading to high N 2 O emission peaks after N fertilizer applications. These peaks contributed greatly to the annual N 2 O emissions in the crop rotation. A significant correlation between annual N 2 O emissions and narG, nirS , and nirK gene numbers indicates that the abundance of these genes is related to N 2 O emission under conditions for denitrification, thus partly contributing to the annual N 2 O emissions. These findings will help to draw up appropriate measures for mitigation of N 2 O emissions in this ‘hotspot’ region.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:2045-2322
2045-2322
DOI:10.1038/srep43283