CpMAX1a , a Cytochrome P450 Monooxygenase Gene of Chimonanthus praecox Regulates Shoot Branching in Arabidopsis

• Published in: International journal of molecular sciences Vol. 23; no. 18; p. 10888
• Main Authors: Zhang, Haiyuan, Hua, Run, Wang, Xia, Wu, Huafeng, Ou, Hua, Lu, Xin, Huang, Yan, Liu, Daofeng, Sui, Shunzhao
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 17.09.2022; MDPI
• Subjects: Arabidopsis; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Binding sites; Biosynthesis; branching; Calycanthaceae; Carotenoids; Chimonanthus praecox; Chloroplasts; CpMAX1a; Cytochrome; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; Cytochrome P450; Cytochrome P450 monooxygenase; Cytochromes P450; Gene Expression Regulation, Plant; Genes; Genotype & phenotype; Homology; Hormones; Hormones - metabolism; Lactones - metabolism; Localization; Metabolites; Phenotypes; Phylogenetics; Plant resistance; Plant Shoots - metabolism; Proteins; Rosette; Stems; strigolactones; wintersweet; wintersweet; CpMAX1a; branching; strigolactones
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms231810888

Strigolactones (SLs) are a class of important hormones in the regulation of plant branching. In the model plant Arabidopsis, AtMAX1 encodes a cytochrome P450 protein and is a crucial gene in the strigolactone synthesis pathway. Yet, the regulatory mechanism of MAX1 in the shoot branching of wintersweet (Chimonanthus praecox) remains unclear. Here we identified and isolated three MAX1 homologous genes, namely CpMAX1a, CpMAX1b, and CpMAX1c. Quantitative real-time PCR (qRT-PCR) revealed the expression of CpMAX1a in all tissues, being highest in leaves, whereas CpMAX1b was only expressed in stems, while CpMAX1c was expressed in both roots and stem tips. However, CpMAX1a’s expression decreased significantly after decapitation; hence, we verified its gene function. CpMAX1a was located in Arabidopsis chloroplasts. Overexpressing CpMAX1a restored the phenotype of the branching mutant max1−3, and reduced the rosette branch number, but resulted in no significant phenotypic differences from the wild type. Additionally, expression of AtBRC1 was significantly upregulated in transgenic lines, indicating that the CpMAX1a gene has a function similar to the homologous gene of Arabidopsis. In conclusion, our study shows that CpMAX1a plays a conserved role in regulating the branch development of wintersweet. This work provides a molecular and theoretical basis for better understanding the branch development of wintersweet.