The fabrication of protein chip based on surface plasmon resonance for detection of pathogens

Protein chip based on surface plasmon resonance (SPR) was developed for detection of pathogens existing in contaminated environment, such as Escherichia coli O157:H7, Salmonella typhimurium, Legionella pneumophila, and Yersinia enterocolitica. Protein G was immobilized to endow the orientation of an...

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Bibliographic Details
Published inBiosensors & bioelectronics Vol. 20; no. 9; pp. 1847 - 1850
Main Authors Oh, Byung-Keun, Lee, Woochang, Chun, Bum Suk, Bae, Young Min, Lee, Won Hong, Choi, Jeong-Woo
Format Journal Article
LanguageEnglish
Published Lausanne Elsevier B.V 15.03.2005
Elsevier Science
Subjects
Bacteria - immunology
Bacteria - isolation & purification
Biocompatible Materials - chemistry
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Biotechnology
Equipment Design
Equipment Failure Analysis
Escherichia coli
Fundamental and applied biological sciences. Psychology
Immunoassay - instrumentation
Immunoassay - methods
Legionella pneumophila
Materials Testing
Methods. Procedures. Technologies
Nerve Tissue Proteins - chemistry
Nerve Tissue Proteins - immunology
Pathogen
Protein Array Analysis - instrumentation
Protein Array Analysis - methods
Protein chip
Protein G
Salmonella typhimurium
Self-assembly technique
Surface plasmon resonance
Surface Plasmon Resonance - instrumentation
Surface Plasmon Resonance - methods
Various methods and equipments
Yersinia enterocolitica
Protein chip
Surface plasmon resonance
Self-assembly technique
Pathogen
Protein G
Self assembly
Biochip
Surface plasmon
Biosensor
Pathogenic
Resonance
Technique
Detection
G protein
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Summary:Protein chip based on surface plasmon resonance (SPR) was developed for detection of pathogens existing in contaminated environment, such as Escherichia coli O157:H7, Salmonella typhimurium, Legionella pneumophila, and Yersinia enterocolitica. Protein G was immobilized to endow the orientation of antibody molecules on the SPR surface. The pathogen binding of the protein chip was investigated by SPR spectroscopy. Consequently, it was found that the four kinds of pathogen could be selectively detected by using SPR-based protein chip.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2004.05.010